投喂水平对快速生长转基因鲤能量分配与越冬的影响

发布时间：2018-06-18 20:25

本文选题：快速生长 + 转基因鲤　；参考：《农业生物技术学报》2017年09期

【摘要】：转草鱼(Ctenopharyngodon idellus)生长激素基因鲤(Cyprinus carpio)是一种以提高生长速度为目标的鱼类新品种,但是转基因可能改变了转基因鲤对不同环境条件的响应对策。为了探讨投喂水平对快速生长转基因鲤能量分配和越冬存活的影响,本研究在两种投喂水平(饱食和半饱食)下开展了对转基因鲤和对照鲤的生长和越冬实验。在夏秋季生长实验中,饱食投喂转基因鲤(satiation transgenic,ST)的体重生长速度是饱食投喂对照鲤(satiation nontransgenic,SN)的1.33倍,半饱食投喂转基因鲤(half-satiation transgenic,HT)的体重生长速度则是半饱食投喂对照鲤(half-satiation nontransgenic,HN)的1.51倍,但是HT和SN组的体重生长速度没有显著性差异。HN,SN,HT和ST组的水分含量依次减小。同一基因型不同投喂水平下的蛋白含量没有显著性差异,但ST组的蛋白含量显著高于SN组。HN,SN,HT和ST组的脂肪含量依次显著增加。在秋季末期,对照鲤的饱食水平明显下降,而转基因鲤的饱食水平并没有明显下降。各组实验鱼的越冬存活率无显著性差异,投喂史只对越冬后的能量含量有显著性影响。本研究表明,转基因鲤和对照鲤均能对投喂水平和越冬做出一致的能量分配响应,限制投喂能激发转基因鲤优先保障生长能量供应而减少能量的存储,转基因鲤和对照鲤的越冬存活能力没有明显差异。本研究结果可为转基因鲤生态安全评估提供参考。
[Abstract]:Cyprinus Carpio, the growth hormone gene of Ctenopharyngodon idellus, is a new fish variety with the aim of increasing growth rate, but it may change the response of transgenic carp to different environmental conditions. In order to investigate the effect of feeding level on energy distribution and overwintering survival of transgenic carp, the growth and wintering of transgenic carp and control carp were studied under two feeding levels (full and semi-full). In the summer and autumn growth experiments, the growth rate of body weight fed with full food was 1.33 times that of the control fed with satiation nontransgenicl (SNN), and 1.51 times that with half-satiety fed with half-satiation of transgenic common carp (HTH), compared with half-satiation fed on half-satiation nontransgenetic HN of the control. However, there was no significant difference in body weight growth rate between HT and SN groups. The protein content of St group was significantly higher than that of SN group, and the fat content of St group was significantly higher than that of SN group and St group. At the end of autumn, the satiety level of the control carp decreased significantly, but the satiety level of the transgenic carp did not. There was no significant difference in the survival rate of overwintering fish in each group, but only the energy content after overwintering was significantly affected by feeding history. The results showed that both transgenic carp and control carp could make consistent energy distribution response to the level of feeding and overwintering, and the restriction of feeding could stimulate transgenic carp to protect the energy supply of growth and reduce the storage of energy. There was no significant difference in survival ability between transgenic carp and control carp. The results of this study can provide a reference for ecological safety assessment of transgenic carp.
【作者单位】：中国科学院水生生物研究所淡水生态与生物技术国家重点实验室;中国水产科学研究院长江水产研究所农业部淡水生物多样性保护重点实验室;
【基金】：国家自然科学基金(No.31200423和No.30970553) 农业部公益性行业科研专项经费项目(No.201203081)
【分类号】：S917.4

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