高碱环境下青海湖裸鲤氮废物排泄及相关基因的表达规律

发布时间：2018-06-18 21:27

本文选题：青海湖裸鲤 + 碳酸盐碱度　；参考：《中国水产科学》2017年04期

【摘要】：采用个体生理学及定量PCR方法,研究了青海湖裸鲤(Gymnocypris przewalskii)在32 mmol/L(CA32)和64 mmol/L(CA64)碳酸盐碱度胁迫下氮废物排泄规律及鳃和肾组织中Rhesus type b glycoproteins(Rhbg)、Rhesus type c2 glycoproteins(Rhcg2)与urea transporter(Ut)基因的表达规律。结果显示,在32 mmol/L碱度胁迫整个过程中及64 mmol/L碱度胁迫初期裸鲤氨氮排泄率显著降低,但在64 mmol/L碱度胁迫8~20 h、24~72 h时氨氮排泄率基本恢复到胁迫前水平,在32 mmol/L碱度胁迫12~16 h、20~24 h及64 mmol/L碱度胁迫16~48 h时尿素氮排泄率显著升高。定量PCR结果显示,Rhbg、Rhcg2、Ut基因在胁迫过程中都有表达上调趋势,其中32 mmol/L碱性环境下鳃组织中Rhbg基因在胁迫12 h时表达明显上调;64 mmol/L碱性环境下鳃组织中Rhcg2基因在胁迫6 h、48 h、72 h表达明显上调,Ut基因在胁迫6 h表达明显上调,肾组织中Rhcg2基因在胁迫6 h表达明显上调。以上结果表明,裸鲤在高碱环境下虽然前期氮废物排泄受到抑制,但后期会通过启动Rh基因高表达恢复氨氮排泄,同时启动Ut高表达来增加尿素氮排泄来进行氮废物排泄。这一特殊氮废物排泄策略有助于裸鲤更好地适应高碱性环境。
[Abstract]:By means of individual physiology and quantitative PCR, the regulation of nitrogen excretion under carbonate alkalinity stress of Gymnocypris przewalskii (32 mmol / L LCA32) and 64 mmol / L CA64) and the expression of Rhesus type b glycoproteinsRhcg2 and urea transporterus Ut2 gene in Gymnocypris c2 glycoproteinsRhcg2 and Rhesus type c 2 glycoproteins Rhcg2) in Gymnocypris c2 and 64 mmol / L LCA64 in Qinghai Lake were studied. The results showed that during the whole process of 32 mmol / L alkalinity stress and at the initial stage of 64 mmol / L basicity stress, the ammonia nitrogen excretion rate of naked carp decreased significantly, but the ammonia nitrogen excretion rate returned to the pre-stress level after 64 mmol / L alkalinity stress for 820 h or 2472 h. The urea nitrogen excretion rate increased significantly under 32 mmol / L alkalinity stress for 24 h and 64 mmol / L basicity stress for 1648 h. The results of quantitative PCR showed that the expression of Rhcg2Ut gene was up-regulated during stress. The expression of Rhbg gene in Gill tissues of 32 mmol / L alkaline environment was upregulated significantly at 12 h of stress and the expression of Rhcg2 gene in Gill tissue of Gill tissue was obviously upregulated at 6 h, 48 h and 72 h after 6 h stress, and the expression of Ut gene was obviously up-regulated at 6 h under the stress of 64 mmol / L alkaline environment. The expression of Rhcg2 gene in renal tissue was up-regulated 6 h after stress. The above results indicated that although nitrogen waste excretion was inhibited in the early stage, the ammonia nitrogen excretion was recovered in the later stage by activating the high expression of Rh gene, and the urea nitrogen excretion was increased by the high expression of Ut in the high alkali environment. This special nitrogen waste excretion strategy helps naked carp to better adapt to high alkaline environment.
【作者单位】：中国水产科学研究院东海水产研究所盐碱水域渔业工程技术研究中心(上海);上海海洋大学水产与生命学院;青海湖裸鲤救护中心;
【基金】：上海市科学技术委员会科研计划项目(14ZR1449800) 中央级公益性科研院所基本科研业务费专项(中国水产科学研究院东海水产研究所)(2012M05)
【分类号】：S917.4

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