转基因油菜Ms8质粒分子的制备

发布时间：2018-06-18 23:22

  本文选题：转基因油菜 + 数字PCR　； 参考：《浙江农业学报》2017年05期

【摘要】：随着转基因产品的大规模商业化和我国大规模进口油菜籽,有效管控存在的风险显得非常必要和有意义,试验研究了质粒标准分子pMs8的研制过程,包括质粒分子的构建、纯度分析、特异性检测、均匀性检验、稳定性考察、定值。结果表明,该质粒分子标准物质的内源基因和外源基因的均匀性良好,没有扩增出所选的其他特异性基因,短期稳定性在14 d(处理温度大于25℃)发生显著变化,长期稳定性大于6个月,两种方法对外源基因拷贝数的定值结果相近,因此,p Ms8是有准确量值的质粒标准分子。可替代性研究结果表明,其可替代基因组作为转基因油菜Ms8定量的阳性标准。
[Abstract]:With the large-scale commercialization of transgenic products and the large-scale import of rapeseed in China, it is necessary and meaningful to effectively control the risks. The development process of plasmid standard molecule pMs8, including the construction of plasmid molecule, is studied experimentally. Purity analysis, specificity test, homogeneity test, stability test, fixed value. The results showed that the homogeneity of endogenous gene and exogenous gene of the molecular standard material of the plasmid was good, no other specific genes were amplified, and the short-term stability changed significantly at 14 days (treatment temperature > 25 鈩，

本文编号：2037233