基于转录组测序挖掘商陆皂苷甲生物合成相关基因

发布时间：2018-06-20 18:08

本文选题：商陆 + 商陆皂苷甲　；参考：《药学学报》2017年09期

【摘要】：为研究商陆皂苷甲的生物合成途径,利用Illumina Hi Seq 4000高通量测序技术对商陆幼苗进行转录组测序,得到9.60 Gb clean data,经Trinity软件组装后获得63 957条unigenes,平均长度988.82 bp,其中24 517条unigenes(38.33%)能被Nr、Swiss-Prot、COG、KOG、Pfam、GO、KEGG等公共数据库注释。对注释得到的unigenes进行KEGG代谢通路分析,发现商陆转录组中有53个unigenes参与萜类骨架合成通路,有8个unigenes参与三萜合成通路,还有417个unigenes参与商陆其他次生代谢途径。进一步分析参与商陆皂苷甲生物合成后修饰酶相关基因,发现有130个unigenes可能具有CYP450的功能,参与商陆次生代谢产物的氧化/羟基化修饰;有46个unigenes与糖基转移酶UGT相关。商陆转录组数据的获得为研究商陆皂苷甲和其他次生代谢产物的生物合成途径奠定了基础,也为商陆药材品质的形成提供理论依据。
[Abstract]:In order to study the biosynthesis pathway of pokeweed saponin A, high throughput sequencing technique of Illumina Hi Seq 4000 was used to sequence the transcription sequence of pokeweed seedlings. After being assembled by Trinity software, we got 63,957 unigeneses, with an average length of 988.82 BP, of which 24,517 unigeneses (38.33) can be annotated by NrSwiss-ProtCOGGPfamKEGG and other public databases. KEGG metabolic pathway analysis showed that 53 unigenes were involved in terpenoid skeleton synthesis, 8 unigenes were involved in triterpenoid synthesis, and 417 unigenes were involved in other secondary pathways of pokeweed transcription. Further analysis of the genes involved in poke-saponin A biosynthesis enzyme showed that 130 unigenes may have CYP450 function and participate in the oxidative / hydroxylation modification of secondary metabolites of pokeweed, and 46 unigenes were related to glycosyltransferase UGT. The obtained data of pokeweed transcription set up the basis for studying the biosynthesis pathway of pokeweed saponin A and other secondary metabolites, and also provided the theoretical basis for the formation of the quality of pokeweed.
【作者单位】：河南中医药大学药学院;呼吸疾病诊疗与新药研发河南省协同创新中心;
【基金】：中央引导地方科技发展专项(河南道地大宗药材种质评价及集约化种植与示范) 教育部科学技术研究重点资助项目:伏牛山中药资源区系分析研究(DF2003078) 河南省科技攻关计划资助项目(162102310468)
【分类号】：S567.239

【相似文献】