柳树β微管蛋白基因家族的克隆和序列分析

发布时间：2018-06-21 03:26

本文选题：柳树 + β微管蛋白　；参考：《西北植物学报》2016年05期

【摘要】：该研究克隆鉴定了旱柳和龙爪柳β微管蛋白基因,并对其进行了序列相似性、系统发育、染色体定位以及表达模式的分析。结果显示,2种柳树β微管蛋白基因家族各有20个成员,家族内部成员间核酸和氨基酸序列相似性分别在74.0%和86.6%以上,种间同源蛋白氨基酸序列相似性在85.8%以上,柳树与其它植物β微管蛋白间的氨基酸序列相似性在81.5%以上。系统发育分析显示,柳树β微管蛋白家族被分为4个亚组,结合杨树β微管蛋白基因染色体定位,推测柳树β微管蛋白基因家族经历了杨柳科全基因组重复事件和串联重复事件,而柳树TUB11和TUB12可能来源于区段重复或者转座。基因表达模式分析发现,该家族成员的表达具有一定的组织特异性,并且部分重复基因对在所检测组织中表达差异较大。柳树β微管蛋白基因家族成员序列的高度相似性、成员数量的进化扩张、以及表达模式的多样性可能赋予了细胞分裂与生长更高的灵活性,这对多年生木本植物的生长发育习性意义重大。
[Abstract]:In this study, the 尾 tubulin genes were cloned and identified, and their sequence similarity, phylogeny, chromosome location and expression pattern were analyzed. The results showed that there were 20 members of 尾 -tubulin gene family in two species of willow, and the similarity of nucleic acid and amino acid sequence was 74.0% and 86.6%, respectively, and the amino acid sequence similarity of homologous protein among species was more than 85.8%. The amino acid sequence similarity of 尾 tubulin between willow and other plants was more than 81.5%. Phylogenetic analysis showed that the 尾 -tubulin family of willow was divided into four subgroups, which combined with chromosome localization of poplar 尾 -tubulin gene. It is inferred that the 尾 tubulin gene family of willow experienced the whole genome repeat event and tandem repeat event of willow, while TUB11 and TUB12 of willow probably originated from segment repeat or transposition. The analysis of gene expression pattern showed that the expression of the family members had a certain tissue specificity, and some of the repeated gene pairs expressed differently in the tested tissues. The high similarity of the family of 尾 -tubulin genes, the evolutionary expansion of the number of members, and the diversity of expression patterns may give cells greater flexibility in cell division and growth. This is of great significance to the growth and development habits of perennial woody plants.
【作者单位】：国家林业局林木培育重点实验室中国林业科学研究院林业研究所;南京林业大学南方现代林业协同创新中心;
【基金】：国家自然科学基金青年项目(31400569)
【分类号】：S792.12

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