芥菜型油菜多室基因Bjmc2的精细定位

发布时间：2018-06-21 20:48

  本文选题：芥菜型油菜 + 多室角果　； 参考：《作物学报》2016年12期

【摘要】：芥菜型多室油菜的产量比普通两室油菜更高,定位乃至克隆多室基因可为油菜遗传改良及解释多室角果形成机制创造条件。本研究通过验证JD11-2家系衍生群体仅在BjMc2位点上存在差异,可用于BjMc2的定位。采用AFLP结合BSA法分析BC_5和BC_6群体,筛选到1个与BjMc2连锁的AFLP标记并转化为SCAR标记SC1。基于该AFLP标记序列信息,利用白菜同源序列设计SSR引物和SCAR引物,获得11对SSR标记和1对SCAR标记。通过在芥菜型油菜BAC文库中的挑选,获得2个覆盖目标区域的单克隆,由此开发1个SSR标记。将获得的SCAR和SSR标记扫描BC7群体,构建了两室性状基因BjMc2的遗传连锁图,两侧最近标记ZX17和BACsr96与目标基因之间的遗传距离分别为0.048 cM和0.340 cM,并定位到白菜A7 Scaffold 000019的946~1014 kb之间,约68 kb物理距离。
[Abstract]:The yield of mustard multi-compartment rapeseed was higher than that of common two-compartment rape. Mapping and even cloning of multi-compartment genes could create conditions for genetic improvement and explanation of the formation mechanism of multi-compartment pods in Brassica napus. In this study, it was verified that only BjMc2 loci were different in JD11-2 family derived populations, which could be used to localize BjMc2. An AFLP marker linked to BjMc2 was screened and transformed into scar marker SC1 by AFLP combined with BSA method. Based on the AFLP marker sequence information, SSR primers and scar primers were designed by using the homologous sequence of Chinese cabbage, and 11 pairs of SSR markers and 1 pair of scar markers were obtained. By selecting the BAC library of Brassica napus, two monoclonal clones covering the target region were obtained and an SSR marker was developed. The BC7 population was scanned with scar and SSR markers, and the genetic linkage map of the two-compartment trait gene BjMc2 was constructed. The genetic distances between the two most recent markers ZX17 and BACsr96 and the target gene were 0.048 cm and 0.340 cm, respectively, and the genetic distance was between 9461014 kb and 9461014 kb of Chinese cabbage A7 Scaffold 000019. About 68 kb physical distance.
【作者单位】： 华中农业大学作物遗传改良国家重点实验室/国家油菜工程技术研究中心;
【基金】：国家自然科学基金项目(31571698)资助~~
【分类号】：S565.4

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