金堂黑山羊FSH信号调节对卵巢基因表达谱影响的研究

发布时间：2018-06-22 13:20

本文选题：金堂黑山羊 + FSH信号　；参考：《西南农业学报》2017年11期

【摘要】：【目的】本研究旨在搞清楚金堂黑山羊FSH信号调节机制的作用模式。【方法】将10只生殖周期相同的健康空怀的金堂黑山羊成年母羊随机分成2组,一组为对照组,一组为试验组。在发情后的第2天对试验组金堂黑山羊进行FSH肌肉注射100IU,并在注射24 h后,摘取金堂黑山羊的卵巢;对照组发情后的第3天摘取卵巢。分别提取它们的总RNA,进行表达谱测序。【结果】经测序分析:对照组和试验组分别得到有效序列30 906 038和27 763 272条;经统计分析后,得到归一化序列30 216 946和27 124882条,与山羊基因组的符合率分别达85.30%和86.13%。比对后发现239个基因出现显著性差异表达,其中139个基因上调了表达,100个基因下调了表达。GO功能分类发现在86个生物学过程、细胞组分、分子功能出现了显著性差异。KEGG功能分类发现有6个调节途径发生了显著性改变。【结论】FSH信号强度变化作用在卵巢上主要通过调节卵巢激素分泌、调节卵巢上FSH信号传导通路、调节脂代谢来响应,从而调节了动物的繁殖性能。
[Abstract]:[objective] to study the mechanism of FSH signal regulation in Jintang Black Goat. [methods] Ten healthy, empty pregnant adult ewe of Jintang Black Goat with the same reproductive cycle were randomly divided into two groups, one group was the control group. One group was an experimental group. On the second day after estrus FSH was injected intramuscularly into Jintang black goats and the ovaries of Jintang black goats were removed 24 hours after injection and 3 days after estrus in control group. The total RNAs were extracted and their expression profiles were sequenced. [results] by sequencing analysis, the effective sequences of 30 906 038 and 27 763,272 effective sequences were obtained in the control group and the experimental group respectively, and the normalized sequences were 30 216 946 and 27 124882 after statistical analysis. The coincidence rates with goat genome were 85.30% and 86.13%, respectively. After comparison, 239 genes were found to be differentially expressed, of which 139 genes were up-regulated, and 100 genes were down-regulated. The functional classification of .go was found in 86 biological processes and cell components. There were significant differences in molecular function. KEGG functional classification showed that there were significant changes in six regulatory pathways. [conclusion] the changes of signal intensity of FSH mainly regulate the secretion of ovarian hormones in the ovary. Regulation of FSH signal transduction pathway in ovary and regulation of lipid metabolism in response regulate the reproductive performance of animals.
【作者单位】：四川省畜牧科学研究院;动物遗传育种四川省重点实验室;
【基金】：四川省应用基础研究项目“金堂黑山羊FSH信号通路调节机制的研究”(2015JY0008)
【分类号】：S827

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