抗病基因同源序列的克隆及其在作物中的应用研究进展

发布时间：2018-06-23 13:51

本文选题：抗病基因同源序列 + RGA法　；参考：《基因组学与应用生物学》2017年08期

【摘要】：抗病基因同源序列克隆法即RGA法是克隆抗病基因快速有效的途径。它是根据已克隆植物抗病基因的保守结构域设计简并引物,扩增获得RGAs,通过分析RGAs与抗病基因的关系,确定候选基因,最终获得抗病基因。目前克隆的RGAs主要是根据NBS-LRR和STK两种保守结构域获得。本综述介绍了抗病基因的分类、特征及作用,论述了抗病基因同源序列的克隆及其在遗传图谱构建,基因定位、克隆,植物起源进化及多样性方面的应用及研究进展,为进一步克隆抗病基因、揭示抗病信号途径及抗病机制奠定基础。
[Abstract]:RGA method is a rapid and effective way to clone disease resistance genes by homologous sequence cloning. According to the conserved domain of cloned plant disease resistance genes, degenerate primers were designed to amplify and obtain RGAs. by analyzing the relationship between RGAs and disease-resistant genes, the candidate genes were identified, and the disease-resistant genes were finally obtained. At present, the cloned RGAs were obtained from two conserved domains of NBS-LRR and STK. In this paper, the classification, characteristics and functions of resistance genes are reviewed. The cloning of homologous sequences of resistance genes and their applications in genetic map construction, gene mapping, cloning, plant origin, evolution and diversity are reviewed. To further clone disease resistance gene, to reveal the signal pathway of disease resistance and disease resistance mechanism.
【作者单位】：陕西理工学院生物科学与工程学院;西北农林科技大学农学院;汉台中学;陕西理工学院体育学院;
【基金】：大学生创新创业项目(2016110) 陕西省教育厅项目(14JK1152) “春笋计划”共同资助
【分类号】：Q943.2

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