基因间插入对水稻农艺性状及刺吸式口器害虫取食选择性与种群密度的影响

发布时间：2018-06-24 07:48

本文选题：水稻 + T-DNA插入　；参考：《中国生物防治学报》2017年01期

【摘要】：本文评价了T-DNA插入对水稻农艺性状与抗虫性、褐飞虱Nilaparvata lugens St?l取食选择性及3种刺吸式口器害虫种群密度的影响,以分析转基因操作及外源基因插入可能引起的非预期效应。结果表明,供试ZD1640-3、ZD1640-19、ZD1640-23和ZD1640-24共4个突变体株系T-DNA插入位点附近基因的表达均不受影响;除个别株系的株高、每穗粒数或结实率有降低外,主要农艺性状参数与受体亲本无显著差异。除ZD1640-24外,突变体各株系上褐飞虱、白背飞虱Sogatella furcifera(Horváth)和黑尾叶蝉Nephotettix bipunctatus(Fabricius)平均种群密度与受体亲本无显著差异;且4个突变体株系上3种害虫种群密度在大多数情况下与受体亲本也无显著差异。与受体亲本相比,突变体株系ZD1640-24对褐飞虱的抗性及取食选择性无显著影响。综合分析认为,T-DNA基因间插入并没有引起水稻发生显著的非预期变化,个别突变体某些考查指标的变化主要来自于个体差异。
[Abstract]:The effects of T-DNA insertion on agronomic traits and insect resistance of rice, feeding selectivity of Nilaparvata lugens Stl and population density of three kinds of sucking insect pests were evaluated in order to analyze the unexpected effects caused by transgenic operation and exogenous gene insertion. The results showed that the expression of genes near the T-DNA insertion site of ZD1640-3 and ZD1640-24 mutant lines were not affected, except for the plant height, grain number per panicle or seed setting rate of individual lines, there was no significant difference between the main agronomic characters and the recipient parents. Except for ZD1640-24, there was no significant difference between the average population density of BPH, Sogatella furcifera (Horv 谩 th) and Nephotettix bipunctatus (Fabricius). The population densities of the three pests in the four mutants were not significantly different from those of the recipient parents in most cases. Compared with the recipient parents, the mutant ZD1640-24 had no significant effect on the resistance and feeding selectivity of brown planthopper. The comprehensive analysis showed that the insertion of T-DNA gene did not cause significant unexpected changes in rice, and the changes of some indexes in individual mutants were mainly due to individual differences.
【作者单位】：浙江大学昆虫科学研究所/水稻生物学重点实验室/农业部农业昆虫学重点实验室;
【基金】：国家转基因生物新品种培育重大专项(2014ZX08011-001)
【分类号】：S511;S435.11

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