森林草莓组蛋白去乙酰化酶基因的鉴定和分析

发布时间：2018-06-24 15:44

本文选题：森林草莓 + 去乙酰化　；参考：《南京农业大学学报》2017年02期

【摘要】：[目的]本研究对森林草莓中组蛋白去乙酰化酶基因(HDAC)进行鉴定和分析,并研究其在生长发育以及逆境胁迫响应过程中的表达变化,为深入研究森林草莓组蛋白去乙酰化调控机制奠定基础。[方法]运用生物信息学方法,对森林草莓中HDAC基因进行鉴定,并进行蛋白结构域、染色体定位、基因复制类型和其他进化分析。利用转录组数据分析森林草莓中HDAC基因在不同组织和发育阶段中的表达变化。运用实时荧光定量PCR对森林草莓中HDAC基因在低温、高温胁迫下的表达变化进行分析。[结果]森林草莓中包含12个HDAC基因,其中RPD3/HDA1家族9个,SIR2家族2个,HD2家族1个;RPD3/HDA1家族基因可以分为5类,SIR2家族中基因分为2类。森林草莓中RPD3/HDA1和HD2家族HDAC基因主要通过离散型复制模式进行扩张,SIR2家族在双子叶进化过程中没有发生明显扩张。各个HDAC基因具有发育阶段或组织特异性表达。部分HDAC基因在森林草莓热胁迫处理中有不同程度的响应,对冷胁迫的反应相对较小。[结论]森林草莓HDAC基因具有植物的典型特性,并可能参与了森林草莓的生长发育和对热胁迫的响应。
[Abstract]:[objective] to identify and analyze histone deacetylase gene (HDAC) in forest strawberry, and to study its expression changes during growth and stress response. It lays a foundation for further study on the regulation mechanism of deacetylation of forest strawberry histone. [methods] the HDAC gene in forest strawberry was identified by bioinformatics, and protein domain, chromosome location, gene replication type and other evolutionary analysis were carried out. Transcriptional data were used to analyze the expression of HDAC gene in different tissues and development stages of forest strawberry. The changes of HDAC gene expression in forest strawberry under low temperature and high temperature stress were analyzed by real-time fluorescence quantitative PCR. [results] there were 12 HDAC genes in the forest strawberry. Among them, 9 genes of RPD 3 / HDA1 family, 9 SIR2 family, 2 HD2 family, 1 RPD 3 / HDA1 family gene can be divided into 5 types of SIR2 gene. The RPD3 / HDA1 and HD2 HDAC genes in forest strawberry were mainly expanded by discrete replication model. The SIR2 family did not expand significantly during the evolution of dicotyledon. Each HDAC gene has a developmental stage or tissue specific expression. Some of the HDAC genes responded to different degrees of heat stress in forest strawberry, but the response to cold stress was relatively small. [conclusion] the HDAC gene of forest strawberry has typical plant characteristics and may be involved in the growth and development of forest strawberry and its response to heat stress.
【作者单位】：南京农业大学作物遗传与种质创新国家重点实验室/园艺学院;
【基金】：中央高校基本科研业务费自主创新重点项目(KYZ201605) 南京农业大学SRT项目(1514A04)
【分类号】：S668.4

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