西伯利亚鲟Sox17基因cDNA全长的克隆及其表达分析

发布时间：2018-06-26 23:24

本文选题：西伯利亚鲟 + Sox　；参考：《海洋渔业》2017年06期

【摘要】：采用RACE技术获得西伯利亚鲟(Acipenser baerii)Sox17基因cDNA全长,序列分析表明,西伯利亚鲟Sox17基因cDNA全长2 671 bp,包括588 bp的5'端非翻译区,901 bp的3'端非翻译区和编码393个氨基酸残基的1 183 bp开放阅读框。氨基酸序列比对显示,该基因具较高的保守性,与塞内加尔多鳍鱼(Polypterus senegalus)的相似度最高为72%。系统进化分析表明,西伯利亚鲟与塞内加尔多鳍鱼聚为一支,且支持率为95%。以延伸因子efla(elongation factor 1-alpha)作为内参基因,对Sox17基因在西伯利亚鲟各组织的相对表达量进行分析,发现Sox17基因在不同组织中均有表达,但表达具有明显的组织差异性。在脑中表达量最高,而在肾、血液、眼、肠和胸鳍5个组织中表达量均较低。同时,Sox17基因在西伯利亚鲟不同发育时期均有表达,但表达具有一定的时间差异性。在宽神经板期的表达量高,相当于表达量最低点11日龄仔鱼的167倍。本研究为量化西伯利亚鲟胚胎发育分化过程中相关基因提供了参照工具,同时可为今后深入研究西伯利亚鲟Sox17在胚胎发育过程中的作用提供基础参考资料。
[Abstract]:The full-length cDNA of (Acipenser baerii) Sox17 gene of Acipenser Siberian Sturgeon was obtained by race technique. The full-length cDNA of Sox17 gene of Acipenser Siberia Sturgeon is 2 671 BP, including 588 BP 5'untranslated region 901 BP untranslated region and 1 183 BP open reading frame encoding 393 amino acid residues. Amino acid sequence alignment showed that the gene was highly conserved and had the highest similarity to the Senegalese polyfin fish (Polypterus senegalus). Phylogenetic analysis showed that the Siberian Sturgeon and the Senegalese polyfin were one and the support rate was 95. The relative expression of Sox17 gene in various tissues of Acipenser sibirica was analyzed by using the extension factor efla (elongation factor 1-alpha as internal reference gene. It was found that Sox17 gene was expressed in different tissues, but the expression of Sox17 gene was different in different tissues. The highest expression was found in the brain, but low in the kidney, blood, eye, intestine and pectoral fin. At the same time, Sox17 gene was expressed in different developmental stages of Acipenser sibirica, but the expression had some time difference. The expression level in the broad nerve plate stage was 167 times of that of the 11 day old larva at the lowest level of expression. This study provides a reference tool for quantifying the genes involved in embryonic development and differentiation of Acipenser Siberian Sturgeon, and provides a basic reference for the further study of the role of Sox17 in embryonic development of Acipenser Siberian Sturgeon.
【作者单位】：中国水产科学研究院东海水产研究所农业部东海与远洋渔业资源开发利用重点实验室;上海海洋大学水产与生命学院;
【基金】：国家自然科学基金项目(31302161)
【分类号】：Q78;S917.4

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