基于高通量测序的猪鞭虫雌、雄虫差异基因表达分析

发布时间：2018-06-27 03:45

本文选题：猪鞭虫 + 转录组　；参考：《畜牧兽医学报》2016年09期

【摘要】：为了探明猪鞭虫(Trichuris suis)雌、雄虫的转录组差异,丰富T.suis转录组数据信息,利用Illumina Hiseq2000对临床采集的T.suis雌、雄虫进行高通量测序,将组装得到的Unigenes在NR、GO、COG和KEGG数据库中比对注释,并进行差异表达基因分析。结果,T.suis雌、雄虫分别获得59 657 854条和48 414 184条高质量测序数据,分别组装获得21 026个和28 886个Unigenes。注释到NR、GO、COG和KEGG数据库的雌虫Unigenes数量分别为11 700、2 287、1 650和9 690个,雄虫Unigenes数量分别为12 902、2 414、1 791和10 377个,在NR数据库比对显示84.90%以上信息均来源于猪鞭虫。T.suis雌、雄虫共有4 320个差异表达基因。以雌虫为对照,雄虫中共有3 496个基因表达上调,有824个基因表达下调。共有906个差异表达基因获得160个KEGG通路富集,其中显著富集通路7个。在T.suis转录组中发现了许多重要基因的表达,包括与T.suis繁殖相关的主要精子蛋白、组蛋白H2A/H2B及卵黄蛋白原,与T.suis致病性、免疫及炎症调节相关的蛋白酶、丝氨酸蛋白酶抑制分子和半胱氨酸蛋白酶抑制分子,以及众多未知功能的基因。本研究揭示了T.suis雌、雄虫差异表达基因的数量,获得了这些差异表达基因的功能、分类和代谢通路注释,为丰富T.suis转录组信息,揭示鞭虫与宿主互作特点及抗鞭虫靶点药物研发奠定基础。
[Abstract]:In order to find out the transcriptional differences between male and female Trichuris suis, and to enrich the information of T. suis transcriptional data, we used Illumina Hiseq2000 to carry out high-throughput sequencing of T. suis females and males collected in clinic. The assembled Unigenes were compared and annotated in the NRGO-COG and KEGG databases. The differentially expressed genes were analyzed. Results 59,657,854 and 48,414 184 high-quality sequencing data were obtained from T. suis female and male respectively, and 21,026 and 28,886 Unigenes were obtained respectively. The numbers of female Unigenes and male Unigenes were 11 700 and 9 690, respectively. The numbers of male Unigenes were 12 902 2 414 1 791 and 10 377, respectively. The results of NR database showed that more than 84.90% of the information was from T. T. suis. A total of 4 320 differentially expressed genes were found in males. In female, 3 496 genes were up-regulated and 824 genes were down-regulated. A total of 906 differentially expressed genes were enriched by 160 KEGG pathways, of which 7 were significantly enriched. A number of important genes have been found in T.suis transcriptome, including the major sperm proteins associated with T. suis reproduction, histone H2A / H2B and vitellogen, proteases associated with T. suis pathogenicity, immune and inflammatory regulation. Serine protease suppressor molecules and cysteine protease suppressor molecules, as well as many unknown functional genes. This study revealed the number of differentially expressed genes in T. suis females and males, and obtained the functional, classification and metabolic pathway annotations of these differentially expressed genes in order to enrich the transcriptional information of T. suis. To reveal the characteristics of the interaction between Trichuris and its host and to lay a foundation for the research and development of anti-flagellate target drugs.
【作者单位】：西南大学荣昌校区动物医学系;西南大学动物科技学院;
【基金】：中央高校基本科研业务费专项基金资助项目(XDJK2015C030) 重庆市社会事业与民生保障科技创新专项(CSTC2015SHMSZX80020)
【分类号】：S852.7

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