化浊润燥降气方对食管癌前病变模型小鼠NDRG1蛋白和基因表达的影响

发布时间：2018-06-28 03:30

本文选题：食管癌前病变 + 化浊润燥降气方　；参考：《中国老年学杂志》2017年24期

【摘要】：目的观察化浊润燥降气方对小鼠食管癌前病变组织分化相关基因NDRG1蛋白及基因表达的影响。方法将130只小鼠分为正常组、模型组、阳性对照组、中药预防组(预防组)、中药治疗组(治疗组),采用4-硝基喹啉-氧化物(4-NQO)制备小鼠食管癌前病变模型,采用Western印迹、RT-PCR方法观察各组食管组织中NDRG1蛋白及基因表达。结果 14 w末食管癌前病变模型成功,24 w末实验结束。24 w末模型组小鼠NDRG1蛋白及基因表达水平与正常组相比明显升高(P0.05);阳性对照组、预防组、治疗组分别与模型组相比明显降低(P0.05);预防组、治疗组明显高于阳性对照组(P0.05);预防组较治疗组明显降低(P0.05)。结论 4-NQO是制备小鼠食管癌前病变模型的有效药物。化浊润燥降气方能减少食管组织NDRG1蛋白及基因的表达,延缓小鼠食管癌前病变到食管癌的进程,中药预防用药更具一定优势。
[Abstract]:Objective To observe the effect of the recipe on the expression of NDRG1 protein and gene expression in the tissue differentiation of the precancerous lesion of the esophagus in mice. Methods 130 mice were divided into normal group, model group, positive control group, Chinese medicine prevention group (Prevention Group), Chinese medicine treatment group (treatment group), and 4- nitroquinoline oxide (4-NQO) was used to prepare the precancerous carcinoma of the esophagus in mice. Western blot and RT-PCR method were used to observe the NDRG1 protein and gene expression in the esophageal tissue in each group. Results the precancerous lesion model of 14 W was successful, and the level of NDRG1 protein and gene expression in the.24 W model group was significantly higher than that of the normal group at the end of.24 w (P0.05); the positive control group, the prevention group, and the treatment group were respectively (P0.05). Compared with the model group (P0.05), the prevention group was significantly higher than the positive control group (P0.05), and the prevention group was significantly lower than the treatment group (P0.05). Conclusion 4-NQO is an effective drug for the preparation of the precancerous lesion model of the esophagus in mice. The prescription of the Xun and moistening dryness Qi can reduce the expression of NDRG1 protein and gene in the esophagus tissue and delay the precancerous precancerous disease of the esophagus in mice. Changes in the process of esophageal cancer, Chinese medicine to prevent the use of medicine has certain advantages.
【作者单位】：河北省中医院肿瘤二科;河北医科大学第四医院科研中心;河北省中医院儿科;河北医科大学研究生学院;
【基金】：河北省高层次人才资助项目(No.B2012003007)
【分类号】：R285.5;R-332

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