高光胁迫下坛紫菜定量PCR内参基因的筛选

发布时间：2018-06-28 19:48

本文选题：坛紫菜 + 高光胁迫　；参考：《水产学报》2017年07期

【摘要】：为了筛选不同高光光强胁迫下坛紫菜中表达水平最为稳定的内参基因,本研究采用qRT-PCR技术测定了植物中常用的6种内参基因UBC、TUB、18S、EF2、ACT和GAPDH在不同光照强度下培养的坛紫菜藻体中的绝对表达水平,并采用比较Ct值法、Ge Norm、Bestkeeper和NormFinder软件分析4种方法对6种候选内参基因的表达稳定性进行评估。结果显示,UBC的Ct值变化范围最小,18S的Ct值变化范围最大;ge Norm软件分析结果显示最稳定的内参基因为UBC和EF2;Bestkeeper和NormFinder软件分析结果类似,UBC基因的稳定值M均为最低值(分别为0.044和0.80),说明其表达水平最稳定。研究表明,UBC基因可以作为不同光照条件下坛紫菜基因表达qRT-PCR分析的最适内参基因。
[Abstract]:In order to screen the most stable internal reference gene in porphyra sinensis under different high light intensity stress, In this study, qRT-PCR technique was used to determine the absolute expression levels of six common internal reference genes UBCU, TUBX, TUBN, EF-2ACT and GAPDH in different light intensity cultures of porphyra chinensis. Four methods were used to evaluate the expression stability of six candidate internal reference genes by comparing Ct value method with GE NormBestkeeper and Norm Finder software. The results showed that the range of Ct value of UBC was the smallest and the change range of Ct value of 18s was the largest. The result of software analysis showed that the most stable internal reference group was the most stable because UBC and EF2Bestkeeper and NormFinder software analysis results were similar to the stability value M of UBC gene. It was 0.044 and 0.80, respectively, indicating that its expression level was the most stable. The results showed that UBC gene could be used as the most suitable internal reference gene for qRT-PCR analysis of porphyra chinensis gene expression under different illumination conditions.
【作者单位】：集美大学水产学院;
【基金】：国家“八六三”高技术研究发展计划(2012AA10A411) 国家自然科学基金(41276177) 福建省自然科学基金(2014J05041,2014J07006)~~
【分类号】：S917.3

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