过量表达酿酒酵母ZWF1基因对异丁醇产量的影响

发布时间：2018-06-29 09:54

本文选题：应用生物学 + 酿酒酵母　；参考：《中国科技论文》2017年06期

【摘要】：以W303-1A为出发菌株,过量表达支链氨基酸转氨酶BAT2基因得到工程菌HZAL-2。通过过量表达编码乙酰乳酸合酶的ILV2基因、编码二羟异戊酸脱水酶的ILV3基因,得到工程菌HZAL-2pILV2pILV3。在工程菌HZAL-2pILV2pILV3的基础上进一步过表达编码葡萄糖-6-磷酸脱氢酶的ZWF1基因,得到工程菌HZAL-2pILV2pILV3 22-ZWF1,其异丁醇产量比对照菌株提高6.81倍。结果表明,通过表达ZWF1基因提高NADPH产量可以解决菌体内部还原力不平衡的问题,从而提高异丁醇产量。
[Abstract]:The engineering strain HZAL-2 was obtained by overexpressing BAT2 gene of branched-chain amino acid transaminase (BAT2) with W303-1A as the starting strain. The engineering strain HZAL-2pILV2pILV3 was obtained by overexpressing ILV2 gene encoding acetyllactate synthase and ILV3 gene encoding dihydroxy isovalerate dehydrase. Based on the engineering strain HZAL-2pILV2pILV2pILV3, the ZWF1 gene encoding glucose-6-phosphate dehydrogenase was overexpressed, and the engineering strain HZAL-2pILV2pILV322-ZWF1 was obtained. The yield of isobutanol was 6.81 times higher than that of the control strain. The results showed that increasing NADPH yield by expression of ZWF1 gene could solve the problem of imbalance of reducing power in bacteria and thus increase the yield of isobutanol.
【作者单位】：河北工业大学化工学院;
【基金】：高等学校博士学科点专项科研基金资助项目(20121317120014) 河北省自然科学基金资助项目(B2013202288) 河北省高等学校科学技术研究青年基金资助项目(q2012024)
【分类号】：Q78;Q936

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