小拟南芥ApZFP基因异源超表达促进拟南芥开花并提高耐逆性

发布时间：2018-06-29 16:20

  本文选题：小拟南芥 + 无苞芥　； 参考：《植物学报》2016年03期

【摘要】：锌指蛋白(ZFP)是一类重要的转录因子,广泛参与植物的生长发育和非生物胁迫应答。新疆小拟南芥(Arabidopsis pumila)又名无苞芥,是十字花科短命植物,具有高光效、繁殖力强和适应干旱等生物学特征,而且比模式植物拟南芥(A.thaliana)更耐高盐胁迫。将前期克隆的小拟南芥锌指蛋白基因Ap ZFP通过花滴法转化到哥伦比亚生态型拟南芥(Col-0)中,获得了独立表达的转基因株系。表型观察发现,过量表达Ap ZFP基因可促使拟南芥在长短日照下均提前开花。实时荧光定量PCR结果显示,转基因拟南芥株系中,光周期途径中的CO基因和年龄途径中的SPL基因表达上调;春化、环境温度和自主途径中的FLC基因表达下调;编码成花素的基因FT及下游开花相关基因AP1和LFY的表达量均升高。进一步通过盐、干旱和ABA胁迫处理Ap ZFP转基因株系的种子和幼苗,发现在胁迫处理下,与对照相比,转基因拟南芥种子萌发率较高,幼苗主根较长。因此推测,Ap ZFP在植物发育过程中具有多种功能,可能既参与植物的开花转变过程,又同其它植物的锌指蛋白基因一样,参与植物的耐逆过程。
[Abstract]:Zinc finger protein (ZFP) is an important transcriptional factor which is widely involved in plant growth and abiotic stress response. Xinjiang small Arabidopsis thaliana (Arabidopsis pumila) is a short-lived cruciferous plant with high light efficiency, strong fecundity and drought adaptation, and is more tolerant to salt stress than the model plant, Arabidopsis thaliana (A.thaliana). The previously cloned zinc finger protein gene of Arabidopsis thaliana Ap ZFP was transformed into Colombian ecotypic Arabidopsis thaliana (Col-0) by flower drop method. Phenotypic observation showed that overexpression of Ap ZFP gene promoted the flowering of Arabidopsis thaliana under long and short days. Real-time fluorescence quantitative PCR showed that CO gene expression in photoperiod pathway and SPL gene expression in age pathway were up-regulated in transgenic Arabidopsis thaliana lines, but FLC gene expression was down-regulated in vernalization, ambient temperature and autonomous pathway. The expression of FT and AP1 and LFY genes were increased. The seeds and seedlings of Ap ZFP transgenic lines were further treated with salt, drought and ABA stress. It was found that under stress treatment, the germination rate of transgenic Arabidopsis thaliana seeds was higher and the main root of transgenic Arabidopsis seedlings was longer than that of control. Therefore, it is inferred that Ap ZFP has many functions in plant development, which may not only participate in the process of plant flowering transformation, but also participate in the process of plant stress tolerance, just like the zinc finger protein gene of other plants.
【作者单位】： 石河子大学生命科学学院;
【基金】：国家自然科学基金(No.U1303302,31060149) 石河子大学高层次人才活动项目(No.RCZX200902)
【分类号】：Q943.2
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本文编号：2082679