蒙原羚朊蛋白基因的序列分析和多态性研究

发布时间：2018-06-29 18:42

本文选题：蒙原羚 + 朊蛋白基因　；参考：《野生动物学报》2017年02期

【摘要】：传染性海绵状脑病(TSE)是发生于人和动物神经系统的退行性疾病,影响着人类的健康及动物福利。朊蛋白基因的ORF氨基酸的多态性(Prnp)与TSE的潜伏期、遗传易感性及种间屏障有关。在本研究中,首次对蒙原羚(Procapra gutturosa)朊蛋白基因的ORF进行全序列分析和多态性研究。研究结果发现,26只蒙原羚Prnp高度同源,与汤氏瞪羚(Eudorcas thomsonii)、印度羚(Antilope cervicapra)、牛(Bos taurus)的SNP的同源性较近,分别为100%、100%和98.5%,然而,斑纹角马(Connochaetes taurinus)、欧洲狍(Capreolus capreolus)和绵羊(Ovis aries)与八肽重复区的缺失相关,蒙原羚与它们的同源性分别为99.3%、99.3%和98.9%。蒙原羚在6个位点发生氨基酸的置换分别为119(N→S),143(S→G),160(Y→H),172(V→A),182(N→S)和221(V→A)。在N端的R5(87-94)缺失1个八肽重复区。蒙原羚Prnp序列的特殊性可能影响分子间的相互作用和TSE种间屏障起关键的作用。
[Abstract]:Infectious spongiform encephalopathy (TSE) is a degenerative disease of human and animal nervous system, which affects human health and animal welfare. The polymorphisms of ORF amino acids (Prnp) in prion gene were associated with the latent period of TSE, genetic susceptibility and interspecific barrier. In this study, the ORFs of prion protein gene of Procapra gutturosa were studied for the first time. The results showed that Prnp was highly homologous to Eudorcas thomsonii), (Antilope cervicapra), (Bos taurus), which was 100% and 98.5%, respectively. The deletion of octopeptide repeats in capreolus and ovis aries of Connochaetes taurinus), is associated with the deletion of octapeptide repeats. The homology of Mongolian wildebeest with them is 99.3% and 98.9%, respectively. The amino acid substitutions at the 6 loci were 119 (Nos) 143C (S G) 160 (Y) H) 172 (VBA) 182 (Nos) and 221 (V A).), respectively. An octapeptide repeat region was deleted in N terminal R 5 (87 94). The particularity of Prnp sequence may influence the intermolecular interaction and the TSE interspecific barrier.
【作者单位】：内蒙古二连浩特出入境检验检疫局;中国检验检疫科学研究院;中国农业大学动物医学院国家动物海绵状脑病实验室;
【分类号】：Q78

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