甘蓝型油菜盐胁迫下幼苗鲜重和干重QTL定位及候选基因分析

发布时间：2018-06-30 16:55

本文选题：甘蓝型油菜 + 盐胁迫　；参考：《作物学报》2017年02期

【摘要】：盐胁迫是非生物胁迫中影响作物产量的一个主要因素,利用分子标记方法选育油菜耐盐品种对提高油菜产量具有重要意义。选用来自GH06与P174杂交后通过单粒传法连续自交获得的高世代重组自交系群体,以含16 g L 1Na Cl的Hoagland溶液培养幼苗进行盐胁迫处理25 d后,分别测定叶和根的鲜重及干重,根据已构建的高密度SNP遗传连锁图谱进行QTL定位,在QTL物理区间筛选耐盐相关基因并以极端表型材料进行q RT-PCR分析。采用复合区间作图法(CIM),在对照和盐胁迫处理中共检测到19个QTL,其中与盐胁迫相关的有6个,可解释的表型变异7.16%~16.15%,分布在A02、A04和C03染色体上,将QTL置信区间序列和拟南芥中与盐胁迫相关的基因比对分析,共找到8个候选基因。对其中4个候选基因在极端表型材料中的表达分析表明,BnaA02g14680D与BnaA02g14490D基因在盐胁迫处理后的48 h或72 h表达量均高于对照组,即基因的表达由盐胁迫引起,而BnaC03g64030D在敏感型材料中的相对表达量高于在耐盐型材料中,BnaC03g62830D在敏感型材料中没有明显变化,但在耐盐型材料中呈现先升高后降低的表达特征,其表达可能会增强植株对盐胁迫的耐受力。本研究为油菜耐盐基因功能挖掘和油菜耐盐品种选育奠定基础。
[Abstract]:Salt stress is a major factor affecting crop yield in abiotic stress. It is important to select salt-tolerant rapeseed varieties by using molecular marker method. The high generation recombinant inbred line population, which was obtained by single grain transmission method after hybridization between GH06 and P174, was cultured in Hoagland solution containing 16g L ~ (-1) NaCl for 25 days. The fresh weight and dry weight of leaves and roots were measured. QTL mapping was carried out according to the constructed high density SNP linkage map. The salt-tolerant genes were screened in the physical interval of QTL and analyzed by qRT-PCR with extreme phenotypic materials. Using compound interval mapping (CIM), 19 QTLs were detected in the control and salt stress treatments, of which 6 were associated with salt stress. Phenotypic variation of 7.16 ~ 16.15 was explained on chromosomes A02A04 and C03. QTL confidence interval sequence and salt stress related gene alignment in Arabidopsis thaliana were analyzed and 8 candidate genes were identified. The expression analysis of four candidate genes in the extreme phenotypic materials showed that the expression levels of BnaA02g14680D and BnaA02g14490D were higher than those of the control at 48 h or 72 h after salt stress, that is, the gene expression was induced by salt stress. The relative expression of BnaC03g64030D in sensitive materials was higher than that in salt-tolerant materials, but the expression of BnaC03g64030D in salt-tolerant materials increased first and then decreased. Its expression may enhance plant tolerance to salt stress. This study laid a foundation for function mining of salt-tolerant genes and breeding of salt-tolerant varieties in Brassica napus.
【作者单位】：西南大学农学与生物科技学院;
【基金】：国家自然科学基金项目(31371655)资助~~
【分类号】：S565.4

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