水稻抽穗期基因EHD8的遗传分析及精细定位

发布时间：2018-07-03 04:43

本文选题：作物遗传学 + 水稻　；参考：《中国科技论文》2017年12期

【摘要】：抽穗期是水稻重要的农艺性状之一,挖掘特异等位变异形式的抽穗期相关基因,对于改良水稻抽穗期至关重要。通过EMS诱变籼稻品种9311获得一个稳定遗传的早抽穗突变体ehd8。与野生型9311相比,ehd8在南宁自然长日照和南宁自然短日照条件下,分别提前抽穗6.7d和10.2d。此外,在南宁自然短日照条件下,ehd8的株高和每穗粒数比野生型9311显著降低。遗传分析表明,ehd8早抽穗性状受一对隐性核基因控制。EHD8基因被定位在8号染色体短臂上17kb范围内。基因注释信息表明,该定位区间内只有1个预测的候选基因LOC_Os080174500与已知的抽穗期基因EF8等位。序列比对分析发现,与野生型9311相比,ehd8突变体中LOC_Os080174500编码区的第11位碱基发生了G→T置换,造成其所编码蛋白的第四位氨基酸发生了精氨酸→蛋氨酸的置换。EHD8新等位基因的发掘对于丰富水稻特异种质资源以及促进其在水稻育种中的应用具有重要意义。
[Abstract]:Heading stage is one of the important agronomic traits in rice. It is very important to excavate the genes related to the specific allelic variation for improving the heading stage of rice. A stably inherited early heading mutant ehd8 was obtained by EMS mutagenesis of indica rice variety 9311. Compared with wild type 9311, under the conditions of Nanning natural long sunshine and Nanning natural short day, heading ahead of heading was 6.7d and 10.2d, respectively. In addition, the plant height and the number of grains per panicle were significantly lower than that of wild type 9311 under the condition of natural short sunshine in Nanning. Genetic analysis showed that the early heading traits of EHD8 were controlled by a pair of recessive nuclear genes. The EHD8 gene was located within the 17kb range of the short arm of chromosome 8. Gene annotation indicated that there was only one predicted candidate gene Os080174500 and known EF8 allele in the locus. Sequence alignment analysis showed that G T substitution occurred in the 11th base of the coding region of LOCSP Os080174500 compared with wild type 9311. The discovery of the new allele EHD8 resulting in the replacement of arginine methionine in the fourth amino acid of its encoded protein is of great significance in enriching the specific germplasm resources of rice and promoting its application in rice breeding.
【作者单位】：广西大学农学院;广西大学亚热带农业生物资源保护与利用国家重点实验室;广西大学生命科学与技术学院;
【基金】：高等学校博士学科点专项科研基金资助项目(20134501120004) 广西省自然科学基金资助项目(14GXNSFAA118104)
【分类号】：S511

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