棉花GhMYB146基因克隆及亚细胞定位分析

发布时间：2018-07-03 08:57

本文选题：棉花 + GhMYB　；参考：《核农学报》2016年11期

【摘要】：MYB转录因子是发育、代谢、生物和非生物胁迫应答调控网络中的关键因子。为了分析棉花Gh MYB146转录因子的亚细胞定位,通过RT-PCR和RACE方法从棉花纤维中克隆了1个棉花MYB转录因子Gh MYB146的c DNA序列,采用生物信息学方法分析了Gh MYB146的氨基酸序列,构建了Gh MYB146基因的亚细胞定位载体并进行了亚细胞定位分析。结果表明,克隆的R2R3-MYB基因Gh MYB146的c DNA全长1 027 bp,开放阅读框长879 bp,编码292个氨基酸,蛋白质预测分子量约为33.151 k D,等电点为7.9;推测的氨基酸序列中含有2个高度保守的SANT结构域;Gh MYB146基因组包含3个外显子和2个内含子。进化树分析表明,Gh MYB146和Gh MYB5分为一个分支;亚细胞定位结果表明,Gh MYB146:GFP融合蛋白定位于细胞核中。本试验结果为进一步研究Gh MYB146基因的生物学功能奠定了基础。
[Abstract]:MYB transcription factors are key factors in developmental, metabolic, biological and abiotic stress response regulatory networks. In order to analyze the subcellular localization of cotton Gh MYB146 transcription factor, a cotton MYB transcription factor Gh MYB146 cDNA sequence was cloned from cotton fiber by RT-PCR and race, and the amino acid sequence of Gh MYB146 was analyzed by bioinformatics. The subcellular localization vector of Gh MYB146 gene was constructed and analyzed. The results showed that the cDNA of the cloned R2R3-MYB gene Gh MYB146 was 1 027 BP in length and 879 BP in open reading frame, encoding 292 amino acids. The predicted molecular weight of the protein is about 33.151 KD and the isoelectric point is 7.9. The deduced amino acid sequence contains two highly conserved SANT domain Gh MYB146 genome containing three exons and two introns. Phylogenetic tree analysis showed that Gh MYB146 and Gh MYB5 were divided into one branch, and subcellular localization showed that Gh MYB146: GFP fusion protein was located in the nucleus. The results laid a foundation for further study of the biological function of Gh MYB146 gene.
【作者单位】：新疆农业大学农学院/新疆农业大学农业生物技术重点实验室;中国农业科学院棉花研究所/棉花生物学国家重点实验室;

【相似文献】