脾虚1号方对功能性消化不良脾虚证大鼠胃体组织MLC蛋白与基因表达的影响

发布时间：2018-07-03 16:25

本文选题：脾虚号方 + 功能性消化不良　；参考：《北京中医药大学学报》2017年09期

【摘要】：目的探讨功能性消化不良(FD)脾虚证大鼠胃体组织肌球蛋白轻链(MLC)蛋白与mRNA表达及脾虚1号方的干预作用。方法 60只7 d龄雄性SD大鼠随机分为正常对照组(n=10)、FD脾虚证模型组(n=50)。正常组给予2%蔗糖溶液灌胃,FD脾虚证模型组给予0.1%蔗糖碘乙酰胺溶液灌胃,0.2 m L/(只·d),连续6 d。FD脾虚证模型组正常饲料喂养至6周龄后叠加改良小平台站立,连续14 d。造模结束后随机分为模型组、多潘立酮组、脾虚1号方低、中、高剂量组,各10只,连同正常组,分别给予蒸馏水10 m L/(kg·d)、多潘立酮3.125 mg/(kg·d)、脾虚1号方1.275、2.550、5.100 g/(kg·d),灌胃14 d。采用免疫组化、蛋白质印迹(Western blot)和RT-PCR方法检测胃体组织MLC蛋白及mRNA表达量。结果与正常组相比,免疫组化检测模型组大鼠胃体肌层MLC蛋白定位表达降低;Western blot检测模型组胃体组织MLC蛋白表达量降低;RT-PCR检测模型组大鼠胃体组织MLC mRNA表达量降低,差异均有统计学意义(P0.01)。与模型组相比,免疫组化检测脾虚1号方低、中、高剂量组大鼠胃体肌层MLC蛋白定位表达增加,差异均有统计学意义(P0.05,P0.01);Western blot检测脾虚1号方中、高剂量组大鼠胃体MLC蛋白表达量增加,差异均有统计学意义(P0.01);RT-PCR检测脾虚1号方高剂量组MLC mRNA表达量升高,差异有统计学意义(P0.01)。结论 FD脾虚证模型大鼠存在胃体组织MLC蛋白及基因表达的降低,而脾虚1号方能够上调MLC蛋白及基因的表达。
[Abstract]:Objective to investigate the expression of myosin light chain (MLC) protein and mRNA in gastric body of rats with functional dyspepsia (FD) spleen deficiency syndrome and the intervention effect of spleen deficiency prescription No. 1. Methods 60 male SD rats aged 7 days were randomly divided into normal control group (n = 10) and model group of FD spleen deficiency syndrome (n = 50). The normal group was given 2% sucrose solution by gastric perfusion with FD spleen deficiency syndrome model group. The model group was given 0.1% sucrose iodide acetamide solution intragastric perfusion with 0.2 mL / (d), continuous 6 d.FD spleen deficiency model group). The normal diet was fed to 6 weeks old and the modified small platform was added to stand up for 14 days. After the model was made, the rats were randomly divided into two groups: model group, doperidone group, low, medium and high dose groups, 10 rats in each group, and 10 rats in each group were given distilled water 10 mL / (kg d), doperidone 3.125 mg/ (kg d), spleen deficiency 1 prescription 1.275U 2.550 ~ 5.100g / (kg d), for 14 days. Immunohistochemistry, Western blot and RT-PCR were used to detect the expression of MLC protein and mRNA in gastric body. Results compared with the normal group, the localization expression of MLC protein in the gastric body myometrium of the model group was detected by immunohistochemistry. The expression of MLC protein in the gastric body tissue of the model group was detected by Western blot. The expression of MLC mRNA in the gastric body tissue of the model group was detected by RT-PCR, and the expression of MLC mRNA in the gastric body of the model group was detected by RT-PCR. The difference was statistically significant (P0.01). Compared with the model group, the expression of MLC protein in the gastric muscle layer of the rats in the high dose group was significantly higher than that in the model group. The expression of MLC protein in the gastric body myometrium of the rats in the high dose group was higher than that in the model group, and the difference was statistically significant (P0.05 P0.01). The expression of MLC protein increased in high dose group (P0.01). The expression of MLC mRNA in high dose group (P0.01) was significantly higher than that in high dose group (P0.01). Conclusion there is a decrease in MLC protein and gene expression in gastric body in FD spleen deficiency model rats, while spleen deficiency Formula 1 can upregulate MLC protein and gene expression.
【作者单位】：中国中医科学院西苑医院消化科北京市中医脾胃病研究所;中国中医科学院研究生院;
【基金】：国家重点基础研究发展计划(973计划)资助项目(No.2013CB531703) 国家自然科学基金资助项目(No.81503567) 中国博士后科学基金资助项目(No.2015M1227,2016T90195)~~
【分类号】：R285.5

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