野生大豆花发育相关基因GsLFY的功能研究

发布时间：2018-07-05 02:05

本文选题：野生大豆 + 花发育　；参考：《遗传》2017年01期

【摘要】：LEAFY/FLORICAULA(LFY/FLO)是植物特有的转录因子家族,在控制花器官的诱导与发育中起着重要的作用,但是与野生大豆花发育相关的LFY/FLO同源基因的研究尚未见报道。本研究从野生大豆中克隆获得1个LFY同源基因,命名为Gs LFY,该基因CDS全长1224 bp,包含完整的开放阅读框,编码407个氨基酸。利用实时荧光定量PCR技术对Gs LFY在不同组织中的表达情况进行了分析,结果显示Gs LFY在根、花以及种子中表达,在茎、叶、茎尖中不表达;在花发育的四轮不同器官中(萼片、花瓣、心皮和雄蕊)进行实时荧光定量PCR,结果显示Gs LFY在花萼和雄蕊中表达,在花瓣和心皮中不表达。酵母单杂交实验结果显示,Gs LFY具有转录激活活性。拟南芥原生质体瞬时表达结果表明,Gs LFY定位于细胞核中。转Gs LFY基因烟草植株开花期比对照提前约29天,这为通过分子育种的方法获得花期改变的大豆新品种提供了基因资源和理论基础。
[Abstract]:LEAFY / FLORICAULA (LFY / FLO) is a plant-specific transcription factor family, which plays an important role in controlling the induction and development of floral organs. However, the study of LFY / FLO homologous gene related to wild soybean flower development has not been reported. In this study, a LFY homologous gene named Gs LFY was cloned from wild soybean. The gene contains a complete open reading frame and encodes 407 amino acids with a total length of 1224 BP. The expression of Gs LFY in different tissues was analyzed by real-time fluorescence quantitative PCR. The results showed that Gs LFY was expressed in root, flower and seed, but not in stem, leaf, stem tip, and in four different organs (sepals, sepals). The results showed that Gs LFY was expressed in calyx and stamen, but not in petal and carpels. The results of yeast single hybrid experiments showed that Gs LFY had transcriptional activation activity. The transient expression of Arabidopsis protoplasts showed that Gs LFY was located in the nucleus of Arabidopsis thaliana. The flowering period of LFGs Y transgenic tobacco plants was 29 days earlier than that of the control, which provided genetic resources and theoretical basis for obtaining new varieties with different flowering stages by molecular breeding.
【作者单位】：南京农业大学作物遗传与种质创新国家重点实验室国家大豆改良中心农业部大豆生物学与遗传育种实验室(综合性);
【基金】：国家自然科学基金项目(编号:31371644)资助~~
【分类号】：S565.1;Q943.2

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