桑白皮黄酮提取物对2型糖尿病大鼠非酒精性脂肪肝血管生成相关基因的影响

发布时间：2018-07-06 14:16

本文选题：桑白皮黄酮 + 型糖尿病　；参考：《中国实验方剂学杂志》2017年17期

【摘要】：目的:初步探索桑白皮黄酮提取物对2型糖尿病大鼠非酒精性脂肪肝的治疗作用及机制研究。方法:将24只雄性大鼠随机分为3组,分别为正常组、模型组、桑白皮黄酮组(1.0 g·kg~(-1))。采用腹腔注射小剂量链脲佐菌素(streptozocin,STZ)结合高脂饲料喂养的方法,建立2型糖尿病伴非酒精性脂肪肝的模型,灌胃治疗16周时,观察比较桑白皮黄酮提取物对各组大鼠血清中空腹血糖(FBG),总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),糖耐量(OGTT)变化,油红O染色和苏木素-伊红(HE)染色观察肝脏病理改变,实时荧光定量聚合酶链式反应(Real-time PCR)检测肝脏血管内皮生长因子(vascular endothelial growth factor,VEGF),血小板衍生因子(platelet derived growth factor,PDGF)mRNA的表达。结果:灌胃干预16周后,与正常组比较,模型组大鼠FBG,TC,TG,LDL-C,含量均显著高于正常组(P0.05),应用桑白皮黄酮治疗后,上述生化指标较模型组均有显著下降(P0.05)。糖耐量试验显示模型组胰岛素敏感性下降,应用桑白皮黄酮治疗后胰岛素敏感性较模型组有显著改善。油红O染色结果显示模型组肝细胞胞浆内可见大量红染的脂肪滴,而桑白皮黄酮组肝细胞胞浆内未见红染的脂肪滴。HE染色结果显示模型组肝小叶中肝细胞发生明显的小泡样脂肪变性,部分肝细胞中脂滴融合成大的脂肪空泡,小叶中可见点状坏死,周围有炎性细胞浸润,而桑白皮黄酮组肝细胞结构正常。模型组肝脏中VEGF,PDGF mRNA表达量较正常组有显著升高,应用桑白皮黄酮治疗后,肝脏中VEGF,PDGF mRNA表达量明显降低(P0.05)。结论:桑白皮黄酮提取物对实验性2型糖尿病大鼠非酒精性脂肪性肝有一定的防治作用,其作用机制可能与抑制肝脏中VEGF,PDGF mRNA的表达有关。
[Abstract]:Aim: to explore the therapeutic effect and mechanism of flavonoids extract from Mori Alba L. on non alcoholic fatty liver in type 2 diabetic rats. Methods: Twenty-four male rats were randomly divided into 3 groups: normal group, model group and flavonoid group (1.0 g kg-1). The model of type 2 diabetes with non-alcoholic fatty liver was established by intraperitoneal injection of streptozotocin (STZ) combined with high fat diet. The changes of fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), glucose tolerance (OGTT), oil red O staining and hematoxylin eosin (HE) staining in serum of rats were observed and compared. The expression of vascular endothelial growth factor (vascular endothelial growth) and platelet-derived factor (platelet derived growth) were detected by real-time polymerase chain reaction (real-time PCR). Results: compared with the normal group, the LDL-C content of FBGG TCN in the model group was significantly higher than that in the normal group after 16 weeks of gastric administration (P0.05), and the above biochemical indexes were significantly lower than those in the model group (P0.05). Glucose tolerance test showed that the insulin sensitivity of the model group was decreased, and the insulin sensitivity of the model group was significantly improved after the treatment of mulberry skin flavonoids. The results of oil red O staining showed that a large number of red fat droplets could be seen in the cytoplasm of hepatocytes in the model group. The results of HE staining showed that the hepatic cells in the model group showed obvious vesicular steatosis, and some of the lipid droplets in the liver cells fused into large fat vacuoles. Punctate necrosis and infiltration of inflammatory cells were observed in the lobules, while the hepatocyte structure was normal in the flavonoids group. The expression of VEGF mRNA in liver of model group was significantly higher than that of normal group, and the expression of VEGF PDGF mRNA in liver was significantly decreased after treatment with flavonoids (P0.05). Conclusion: the flavonoids extract from mulberry peel has a preventive and therapeutic effect on non-alcoholic fatty liver in experimental type 2 diabetic rats, and its mechanism may be related to the inhibition of the expression of VEGF PDGF mRNA in the liver.
【作者单位】：广州中医药大学中药学院;
【基金】：国家自然科学基金项目(81373775)
【分类号】：R285.5

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