新疆水稻主要育成品种13个稻瘟病的抗性基因分布

发布时间：2018-07-07 23:18

本文选题：水稻 + 育成品种　；参考：《新疆农业科学》2017年09期

【摘要】：【目的】明确新疆主要育成水稻品种中稻瘟病抗性基因的分布状况,为稻瘟病抗性育种提供依据。【方法】研究以31份新疆选育的水稻品种为材料,利用13个抗稻瘟病基因的特异性分子标记,进行PCR鉴定,初步建立新疆主要育成品种的抗性基因分布情况。【结果】31份供试材料中均无Pi21基因存在;表现为广谱抗性的Pi9、Pita、Pib和Pikm基因所占比例偏少,分别占9.68%、22.58%、6.45%、12.90%;表现为广谱抗性的Pi2、Pigm、Pil、Pikh/Pi54、Pi63和表现为生理小种特异性抗性的Pia、Pid2、Pid3/Pi25基因广泛存在,所占比率67.74%~100%;不同水稻品种抗性基因数量分布在5~11个,含11个抗性基因的品种是新稻23号,其聚合了除Pi21和Pid2外其余所有抗性基因。【结论】新疆主要水稻育成品种中广泛存在具有光谱抗性的Pi2、Pigm、Pil、Pikh/Pi54、Pi63和表现为生理小种特异性抗性的Pia、Pid2、Pid3/Pi25基因,利用特异性分子标记可以有效快速鉴定品种中的抗性基因的存在和分布情况。
[Abstract]:[objective] to determine the distribution of blast resistance genes in main rice varieties in Xinjiang, and to provide a basis for rice blast resistance breeding. [methods] 31 rice varieties selected from Xinjiang were used as materials. Using the specific molecular markers of 13 rice blast resistance genes, the distribution of resistance genes of the main cultivars in Xinjiang was preliminarily established by PCR. [results] there was no Pi21 gene in 31 tested materials. The proportion of Pi9 Pitahe Pib gene and Pikm gene with broad-spectrum resistance is relatively small, accounting for 9.68 ~ 22.58 and 6.455.45 ~ 12.90, respectively; the Pi2Pigmor Pilkh / Pikh-Pi54 Pi63 and the PiaPid2Pid3 / Pi25 / Pi25 gene, which show broad-spectrum resistance, are widely distributed. The number of resistance genes in different rice varieties ranged from 51 to 11, and the variety with 11 resistance genes was Xindao 23. All the resistant genes except Pi21 and Pid2 were polymerized. [conclusion] the spectral resistant Pi2Pigmmor Pilkh-Pikh-Pi54-Pi63 and the Pid2Pid2Pid2 / Pid3 / Pi25 gene were widely found in the main rice cultivars in Xinjiang. Specific molecular markers can be used to identify the existence and distribution of resistance genes.
【作者单位】：新疆农业科学院核技术生物技术研究所;新疆农业科学院温宿水稻试验站;
【基金】：科技部“十三五”七大农作物育种项目课题(2017YFD0100505) 新疆农科院青年基金项目(xjnkq-2015034)~~
【分类号】：S435.111.41

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