大豆GmABCG40基因的功能预测及表达分析

发布时间：2018-07-07 23:36

本文选题：大豆 + ABCG转运蛋白　；参考：《大豆科学》2017年04期

【摘要】：通过在大豆基因组数据库中检索拟南芥At ABCG40在大豆中的同源基因,获得了Gm ABCG40基因序列。通过对Gm ABCG40基因编码的氨基酸序列及启动子序列进行生物信息学分析,结果表明:Gm ABCG40基因CDS序列全长4 284 bp,编码1 427个氨基酸。Gm ABCG40编码的蛋白为疏水性蛋白,具有多个N-糖基化位点、激酶磷酸化位点、N-豆蔻酰化位点、2个ATP/GTP结合位点基序A和1个速激肽家族信号。结构域分析表明Gm ABCG40含有2个核苷酸结合域与2个跨膜结构域,形成NBD1-TMD1-NBD2-TMD2结构,属于ABCG亚家族的成员。Gm ABCG40预测的启动子区域含有与激素、胁迫、光应答、胚乳表达和转录因子结合相关的顺式作用元件。系统进化分析表明Gm ABCG40与菜豆、红豆、木豆、百脉根等豆科植物亲缘关系较近。组织特异性表达分析结果显示Gm DABCG40在叶片中表达量最低,在根中表达量最高,推测其可能参与根中ABA的转运过程。
[Abstract]:By searching the homologous genes of Arabidopsis thaliana at ABCG40 in soybean genome database, the Gm ABCG40 gene sequence was obtained. The amino acid sequence and promoter sequence of Gm ABCG40 gene were analyzed by bioinformatics. The results showed that the whole length of CDS sequence of Gm ABCG40 gene was 4 284 BP, and the protein encoding 1,427 amino acids. Gm ABCG40 was hydrophobic protein. There are many N-glycosylation sites, kinase phosphorylation sites, two ATP / GTP binding site motifs A and a tachykinin family signal. Domain analysis showed that Gm ABCG40 contained two nucleotide binding domains and two transmembrane domains, forming NBD1-TMD1-NBD2-TMD2 structure. Cis-acting elements associated with endosperm expression and transcription factor binding. Phylogenetic analysis showed that GM ABCG40 was closely related to bean, red bean, woody bean, Baimai root and other legume plants. The results of tissue specific expression analysis showed that GmDABCG40 was the lowest in the leaves and the highest in the root, suggesting that GmDABCG40 might be involved in the process of ABA transport in the root.
【作者单位】：东北农业大学农学院/大豆生物学教育部重点实验室;黑龙江省农业科学院生物技术研究所;
【基金】：黑龙江普通本科高等学校青年创新人才培养计划(UNPYSCT-2016005) 抗逆转基因大豆新品培育(2016ZX0804002)
【分类号】：S565.1

【参考文献】