池蝶蚌β-连环蛋白基因cDNA的克隆及表达特征分析

发布时间：2018-07-09 12:02

  本文选题：池蝶蚌 + Hsβ-catenin　； 参考：《水生生物学报》2017年05期

【摘要】：为了解淡水贝类性别调控与分化机制,课题组建立了池蝶蚌(Hyriopsis schlegelii)性腺转录组,在转录组库中,存在β-连环蛋白(β-catenin)基因序列。实验对池蝶蚌β-catenin基因进行验证,采用RACE技术克隆其c DNA全长,命名为Hsβ-catenin。该序列全长4386 bp,5′-非编码区为162 bp,3′-非编码区为1758 bp,开放阅读框为2466 bp,编码821个氨基酸;该蛋白结构域主要由12个ARM重复序列组成;二级结构中,α-螺旋占47.75%,β-折叠占1.22%,随机卷曲占51.04%;三级结构中含大量α-螺旋且为右手超螺旋,构成ARM结构域;系统进化树分析表明,Hsβ-catenin与软体动物聚为一支,然后与昆虫类聚为一支。实时荧光定量PCR(q RT-PCR)检测显示,Hsβ-catenin在肠中表达量最高,其次是斧足和精巢。Hsβ-catenin基因在12月龄和36月龄表达量较高,且在36月龄表达量最高,表明其可能参与池蝶蚌的性别调控与分化作用。
[Abstract]:In order to understand the sex regulation and differentiation mechanism of freshwater shellfish, the gonadal transcriptome of Hyriopsis schlegelii was established. There was a 尾 -catenin gene sequence in the transcriptional library. The cDNA of 尾 -catenin gene was cloned by race technique and named as Hs 尾 -catenin. The total length of the sequence is 4386 BP / 5, and the non-coding region is 162bp / 3G / L, which is 1758 BP and the open reading frame is 2466 BP, which encodes 821 amino acids, and the protein domain is mainly composed of 12 arm repeats. Among the secondary structures, 伪 -helix accounts for 47.75, 尾 -fold for 1.22, random crimp for 51.044.The tertiary structure contains a large number of 伪 -helix and right hand superhelix, which forms the arm domain. Phylogenetic tree analysis shows that Hs 尾 -catenin is one with mollusks and then with insects. The expression of Hs 尾 -catenin was the highest in the intestine by real-time fluorescence quantitative PCR (Q RT-PCR), followed by the expression of Hs 尾 -catenin gene at 12 and 36 months of age and the highest expression at 36 months old. The results suggested that it might be involved in the sex regulation and differentiation.
【作者单位】： 南昌大学生命科学学院;
【基金】：国家自然科学基金(31660337) 江西省教育厅项目(GJJ150166)资助~~
【分类号】：S917.4
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本文编号：2109299