次黄嘌呤核苷酸脱氢酶对猪链球菌2型ZY05719基因转录谱的影响

发布时间：2018-07-09 15:53

本文选题：猪链球菌型 + 表达谱芯片　；参考：《江苏农业科学》2017年06期

【摘要】：次黄嘌呤核苷酸脱氢酶(IMPDH)是猪链球菌2型的毒力相关因子,该基因的丢失降低了猪链球菌2型菌株ZY05719黏附HEp-2、PK15细胞的能力。为全面掌握IMPDH对ZY05719致病力的影响机制,采用定制的Agilent猪链球菌2型基因表达谱芯片,比较了亲本菌株ZY05719(ZY)和impdh敲除菌株(ΔZY)的基因转录情况。结果显示,impdh的丢失影响了254个基因的转录,其中包括impdh的下调基因177个,上调基因77个。对差异基因进行同类群聚类(Clusters of Orthologous Groups,COG)分析,显示差异基因主要集中于氨基酸、碳水化合物的转运代谢,以及核糖体、细胞壁、细胞膜的生物合成和防御机制。本研究较为全面地分析了impdh对猪链球菌2型基因转录谱的影响,为解释impdh在猪链球菌致病中发挥的作用提供了数据支持。
[Abstract]:Hypoxanthine nucleotide dehydrogenase (IMPDH) is a virulence related factor of Streptococcus suis type 2. The loss of this gene reduces the ability of strain ZY05719 to adhere to HEp-2PK15 cells. In order to fully understand the mechanism of the pathogenicity of Agilent on ZY05719, the gene transcription of parent strain ZY05719 (ZY) and impdh knockout strain (螖 ZY) were compared by using a customized Agilent streptococcus suis type 2 gene expression microarray. The results showed that the loss of impdh affected the transcription of 254 genes, including 177 down-regulated genes and 77 up-regulated genes of impdh. The cluster of orthogonal groups cog (COG) analysis showed that differentially expressed genes mainly focus on amino acids, carbohydrate transport and metabolism, as well as ribosome, cell wall, cell membrane biosynthesis and defense mechanisms. In this study, the effect of impdh on the transcription profile of Streptococcus suis type 2 gene was analyzed, which provided data support for explaining the role of impdh in the pathogenicity of Streptococcus suis.
【作者单位】：江苏省农业科学院兽医研究所/农业部兽用生物制品工程技术重点实验室/国家兽用生物制品工程技术研究中心;
【基金】：公益性行业(农业)科研专项(编号:201303041) 江苏省自主创新探索性研究项目(编号:CX[11]2060)
【分类号】：S852.611

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