针刺长强穴对FMR1基因敲除小鼠学习能力及皮质区CREB、BDNF基因表达的影响

发布时间：2018-07-09 21:40

本文选题：CREB + BDNF　；参考：《时珍国医国药》2017年08期

【摘要】：目的比较电针、手针长强穴对FMR1基因敲除小鼠皮质区环磷腺苷效应元件结合蛋白(CREB)mRNA及脑源性神经营养因子(BDNF)mRNA表达量的影响,并探讨其作用机制。方法选取适龄FMR1基因敲除型小鼠18只,随机分为空白组、手针组、电针组,每组6只。取穴长强穴,手针组在留针期间每隔10min行手法运针15s,电针组在手针针刺基础上连接电针仪,施以连续波,频率2Hz,留针30min,每日1次,连续治疗14 d;空白组在同等条件下饲养,并仅模拟抓取,不予任何干预。行为学实验观察小鼠空间学习记忆能力及其自主活动次数,荧光定量PCR检测小鼠皮质区CREB mRNA及BDNF mRNA表达量。结果水迷宫实验中针刺治疗前后小鼠逃避潜伏期具有显著差异(P0.05),且针刺干预后,手针组逃避潜伏期比电针组明显缩短(P0.05),手针组原平台象限时间/逃避潜伏期比电针组明显延长(P0.05)。与电针组比较,手针组皮质区BDNF mRNA表达量明显增加(P0.05);与空白组对比,电针组和手针组皮质区CREB mRNA表达量无明显差异(P0.05);结论针刺"长强"穴,手针组能显著影响FMR1基因敲除小鼠的学习记忆能力及异常行为活动,其机制可能与调控中枢神经的BDNF mRNA、CREB mRNA表达量有关。
[Abstract]:Objective to compare the effects of electroacupuncture and hand acupuncture on the expression of cyclophosphate adenosine effector element binding protein (CREB) mRNA and brain-derived neurotrophic factor (BDNF) mRNA in the cortex of FMR1 gene knockout mice. Methods Eighteen FMR1 knockout mice of appropriate age were randomly divided into blank group, hand acupuncture group and electroacupuncture group with 6 mice in each group. The long and strong points were taken from the acupoints, the hand acupuncture group operated the acupuncture at intervals of 10min every other time for 15 s, the electroacupuncture group was connected with the electroacupuncture instrument on the basis of the hand acupuncture, and the continuous wave was applied at the frequency of 2 Hz, the needle was kept for 30 minutes, once a day for 14 days, and the blank group was fed under the same conditions. And only simulated grab, no intervention. Behavioral experiments were performed to observe the spatial learning and memory ability and the number of autonomic activities in mice. The expressions of CREB mRNA and BDNF mRNA in the cortex of mice were detected by fluorescence quantitative PCR. Results in the water maze experiment, there was significant difference in the escape latency of mice before and after acupuncture treatment (P0.05). After acupuncture intervention, the escape latency of the hand acupuncture group was significantly shorter than that of the electroacupuncture group (P0.05), and the original platform quadrant time / escape latency of the hand acupuncture group was significantly longer than that of the electroacupuncture group (P0.05). Compared with the electroacupuncture group, the expression of BDNF mRNA in the cortex of the hand acupuncture group was significantly increased (P0.05), but there was no significant difference in the expression of CREB mRNA between the electroacupuncture group and the hand acupuncture group (P0.05). The ability of learning and memory and abnormal behavior of FMR1 knockout mice were significantly affected by hand acupuncture group. The mechanism may be related to the regulation of the expression of BDNF mRNA-CREB mRNA in the central nervous system.
【作者单位】：福建中医药大学针灸学院;
【基金】：福建省自然科学基金资助项目(No.2017J01540;No.2017j01540) 福建省卫生系统中青年骨干人才培养项目(No.2014-ZQN-JC-28)
【分类号】：R245

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