鄱阳湖不同植被类型土壤RubisCO酶活及cbbL基因丰度分析

发布时间：2018-07-09 23:02

本文选题：植被类型 + 水位时期　；参考：《江西师范大学》2017年硕士论文

【摘要】：本文分别选取常湖池湿地由湖心至州滩典型植物群落南荻群落(Ass.Triarrhena lutarioriparia)、苔草群落(Ass.Carex Cinerascens)、焚火后区域及裸滩和南矶湿地由州滩至湖心典型植物群落芦苇群落(Ass.Phragmites communis)、南荻群落、苔草群落、芦苇-苔草的混合群落(Ass.Phragmites communis-Carex cinerascens)、茭白群落(Ass.Zizania latifolia)及裸滩为研究对象。分析土壤理化性质、土壤RubisCO酶活、cbbL与16s rDNA丰度的变化特征,结果表明:(1)南矶山土壤总碳、总氮含量不同植被类型间无显著差异(p0.05),且平水期含量均大于枯水期;常湖池南荻土壤总碳、总氮含量显著大于大于裸滩(p0.05),且枯水期枯水期含量均大于丰水期。南矶山和常湖池土壤中脲酶作为第一解释变量,解释土壤酶活在各样方的差异,土壤脲酶活性均呈现南荻苔草裸滩的趋势;且南矶山土壤酶活在不同水位间呈枯水期丰水期平水期的趋势,而在常湖池枯水期和平水期出现了苔草和裸滩土壤酶活较高的异常现象,并没有形成一定趋势。南矶山和常湖池土壤中微生物生物量碳(MBC)作为第一解释变量,解释土壤微生物活性特征在各样方差异,南矶山土壤呈现出南荻苔草裸滩,平水期枯水期≈丰水期的趋势;常湖池平水期和丰水期表现为裸滩南荻苔草,而枯水期呈现南荻苔草裸滩的趋势。植被类型是土壤理化性质发生变化的主要因素,且均呈现南荻苔草的趋势。水位和采样点对土壤理化性质影响较小,在南矶山和常湖池土壤理化性质数值有差异,但解释各性质的解释变量的主次顺序相同。(2)植被覆盖会促进土壤中RubisCO酶活,而水位对酶活产生的影响受植被有无、植被类型及采样点干扰,唯一确定的此干扰的大小及方向是植被覆盖土壤RubisCO酶活在枯水期升高;常湖池土壤RubisCO酶活在不同植被类型间均呈现为焚火南荻苔草裸滩,而南矶山土壤表现为有植被覆盖土壤大于裸滩土壤;南矶山土壤RubisCO酶活在不同水位时期无明显趋势,而常湖池植被覆盖土壤均呈现为枯水期丰水期平水期,裸滩土壤呈为丰水期平水期枯水期。RubisCO影响因子在不同采样点差异较大,相同的主要影响因子为TN、TC和土壤呼吸(REC),在南矶山分别为(r=0.643*、0.5654*、-0.543*),在常湖池分别为(r=-0.854**、-0.743*、-0.674*),在两个采样点,常湖池湿地RubisCO酶活与上述影响因子相关性较大,TN和TC在不同采样地点分别呈正负相关,而REC呈负相关。(3)常湖池土壤cbbL和16s rDNA丰度在平水期呈现为苔草裸滩焚火南荻,枯水期和丰水期呈现为南荻裸滩焚火苔草,南矶山土壤在枯水期呈现为茭白混合≥南荻苔草芦苇裸滩,平水期呈现为南荻混合茭白苔草裸滩,丰水期呈现为苔草芦苇≥茭白南荻裸滩;常湖池丰水期cbbL与16s rDNA丰度均大于枯水期,南矶山不同水位时期cbbL与16s rDNA丰度无明显趋势。在南矶山(r=0.552*,p0.05)和常湖池(r=-0.575*,p0.05)RubisCO酶活与cbbL分别呈正负相关。且在枯水期(r=-0.208,p0.05)、平水期(r=0.368,p0.05)及丰水期(r=0.498,p0.05)也各不相同。而南矶山和常湖池RubisCO酶活与cbb L/16s rDNA比值的相关性分别为(r=0.497、-0.545*,p0.05、p0.05)与RubisCO酶活与cbbL相关性表现一致。
[Abstract]:In this paper , the changes of soil enzyme activity , soil urease activity , soil urease activity and soil urease activity were studied . The results showed that : ( 1 ) There was no significant difference between soil enzyme activity and soil urease activity . The results showed that : ( 1 ) There was no significant difference between soil enzyme activity and soil urease activity . ( 2 ) The vegetation cover can promote the activity of RubisCO enzyme in soil , and the influence of water level on enzyme activity is influenced by the existence of vegetation , vegetation type and sampling point . The only certainty is that the vegetation cover soil is larger than bare beach soil . ( 3 ) The abundance of soil cbbL and 16S rDNA in the common lake basin showed a positive and negative correlation with cbbL during the period of dry season ( r = -0 . 208 , p . 05 ) .
【学位授予单位】：江西师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q948.113

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