新疆褐牛基因外显子多态性及其与SCS和泌乳性状关联性分析

发布时间：2018-07-10 01:52

本文选题：新疆褐牛 + SCS　；参考：《中国农业科学》2017年13期

【摘要】：【目的】探索ERAP2,Cwc27,PPFIBP2,THADA,ZNF804B和AMHR2基因多态性与新疆褐牛体细胞评分及泌乳性状的相关性,旨在寻找与新疆褐牛体细胞评分及泌乳性状相关的分子标记。【方法】以新疆乌鲁木齐种牛场、新疆天山畜牧生物工程股份有限公司共169头新疆褐牛母牛为试验对象,以2008—2015年间的生产性能测定记录(包括乳脂率、乳蛋白率、总固体含量、乳糖率和体细胞计数等)和305 d产奶量为试验数据,基于课题组前期15头中国荷斯坦牛DNA混池重测序的结果筛选外显子区域10个SNPs,采用基质辅助激光电离飞行时间质谱法(sequenom mass Array genotype)分型技术验证10个SNP位点的遗传多态性,运用SAS8.1软件GLM过程对这10个SNP突变与新疆褐牛体细胞评分(SCS)、305 d产奶量、乳脂率、乳蛋白率、乳糖率和总固体的关联程度进行统计分析。【结果】10个SNP位点均具有多态性,χ~2检验表明群体中9个SNP位点符合Hardy-Weinberg平衡状态。关联分析结果表明ERAP2基因两个位点(T98741711C和G98736141A)均与新疆褐牛305 d产奶量达到极显著关联(P0.001),其中TT型和GG型个体的产奶量最高;PPFIBP2(C45667492G)位点与新疆褐牛乳糖率达到显著关联(P0.005),其中GG型个体的乳糖率最高;Cwc27(T14533269A)位点与新疆褐牛乳脂率和总固体含量达到显著关联(P0.005),与SCS达到极显著关联(P0.001),其中TT型个体的乳脂率和总固体含量均最高,AA型个体的SCS极显著高于AT和TT型;AMHR2(C26758055G)位点与新疆褐牛乳蛋白率和总固体含量达到极显著关联(P0.001),其中GG型个体显著高于CC和GC型个体。连锁不平衡分析与单倍型构建的结果发现10个SNPs构建了两个单倍型模块,其中SNP1和SNP2位点之间处于连锁不平衡状态(r20.3),SNP4和SNP10位点之间处于强连锁不平衡状态(r20.6),随后分析了单倍型与SCS和泌乳性状之间的相关性,发现单倍型与新疆褐牛SCS和泌乳性状无显著关联(P0.001)。【结论】初步发现了ERAP2,Cwc27,PPFIBP2和AMHR2与新疆褐牛SCS和泌乳性能相关,结果可为新疆褐牛产奶性状的分子标记辅助选育提供参考和依据。
[Abstract]:[objective] to explore the relationship between the polymorphism of ZNF804B and AMHR2 gene of ERAP2Cwc27PPFIBP2THADAA ZNF804B and AMHR2 gene, and to search for molecular markers related to somatic score and lactation traits of Xinjiang brown cattle. [methods] A cattle farm in Urumqi, Xinjiang, was used to investigate the relationship between the polymorphism of ZNF804B and AMHR2 gene polymorphism and somatic cell score and lactation traits of Xinjiang Brown cattle. Xinjiang Tianshan Animal Husbandry and Bioengineering Co., Ltd., a total of 169 Xinjiang brown cattle cows as experimental objects, the production performance of 2008-2015 records (including milk fat rate, milk protein rate, total solid content, Lactose rate and somatic cell count, etc.) and 305d milk yield were the experimental data. Ten SNPs in exon region were screened based on the resequencing of 15 Chinese Holstein cattle. The genetic polymorphism of 10 SNPs was verified by matrix assisted laser ionization time of flight mass spectrometry (sequenom mass Array genotype) typing. The 10 SNP mutation and SCS 305d milk yield, milk fat rate, milk protein rate were analyzed by using the software GLM of SAS8.1. The correlation between lactose rate and total solids was statistically analyzed. [results] all 10 SNP loci were polymorphic. 蠂 ~ 2 test showed that 9 SNP loci in the population were in Hardy-Weinberg equilibrium state. The results of association analysis showed that the two ERAP2 loci (T98741711C and G98736141A) were significantly correlated with the milk yield of Xinjiang brown cattle (P0.001). The highest milk yield (PPFIBP2 (C45667492G) of the individuals with TT and GG type was significantly correlated with the lactose rate of Xinjiang brown cattle (P0.005). The highest lactose rate of GG individuals was Cwc27 (T14533269A), which was significantly correlated with milk fat rate and total solid content of Xinjiang brown cattle (P0.005), and with SCS (P0.001). The milk fat rate and total solid content of individuals with TT type were the highest in AA individuals. SCS was significantly higher than AT and TT type AMHR2 (C26758055G) loci in Xinjiang brown milk protein rate and total solid content (P0.001), among which GG type individuals were significantly higher than CC and GC type individuals. Linkage disequilibrium analysis and haplotype construction showed that 10 SNPs constructed two haplotype modules. There was strong linkage disequilibrium between SNP1 and SNP2 loci (r20.3) and between SNP4 and SNP10 loci (r20.6). The correlation between haplotypes and SCS and lactation traits was analyzed. It was found that there was no significant correlation between haplotypes and SCS and lactation traits (P0.001). [conclusion] it was found that ERAP2Cwc27 PPFIBP2 and AMHR2 were related to SCS and lactation performance of Xinjiang Brown cattle. The results could provide reference and basis for molecular marker assisted breeding of milk production traits of Xinjiang Brown cattle.
【作者单位】：新疆农业大学动物科学学院;宁夏大学农学院;新疆乌鲁木齐种牛场;新疆天山畜牧生物工程股份有限公司;中国农业大学动物科技学院;
【基金】：“十二五”国家科技支撑计划(2011BAD28B02) 现代农业(奶牛)产业技术体系建设专项资金(CARS-37)
【分类号】：S823

【共引文献】