柑橘黄龙病相关水杨酸羧基甲基转移酶基因CsSAMT-1的克隆与表达分析

发布时间：2018-07-10 11:49

本文选题：柑橘 + 黄龙病　；参考：《园艺学报》2017年12期

【摘要】：为研究水杨酸羧基甲基转移酶(Salicylic acid carboxyl methyltransferase,SAMT)在柑橘防御黄龙病(Huanglongbing,HLB)中的作用,克隆获得Cs SAMT~(-1)基因,并以易感HLB锦橙和耐HLB酸柚为试材进行基因表达分析。q RT-PCR结果表明,易感病锦橙中CsSAMT~(-1)在感染HLB后上调表达,且在叶脉中的表达量显著高于叶肉;而耐病酸柚中CsSAMT~(-1)呈组成型表达。外源水杨酸(SA)和茉莉酸甲酯(MeJA)处理锦橙和酸柚,均可诱导CsSAMT~(-1)上调表达。HLB侵染前,耐病酸柚叶脉中SA和水杨酸甲酯(MeSA)含量分别为11.85和18.18 ng·g~(-1),显著高于锦橙(0.82和0.01 ng·g~(-1));HLB侵染后,锦橙中SA和MeSA含量明显上升,酸柚中SA含量明显下降,MeSA含量基本保持原有水平。研究表明,Cs SAMT~(-1)可能通过调节SA和Me SA信号分子转换来增强对柑橘黄龙病的抗性。
[Abstract]:In order to study the role of salicylic acid carboxyl methyltransferase (SAMT) in the protection of citrus against Huanglongbingang (HLB), the Cs SAMT-1 gene was cloned and the susceptible HLB orange and HLB acid-tolerant grapefruit were used for gene expression analysis. The expression of CsSAMT-1 in susceptible orange was up-regulated after HLB infection, and the expression of CsSAMT-1 in leaf vein was significantly higher than that in mesophyll, while CsSAMT-1 was constitutively expressed in disease-tolerant grapefruit. Exogenous salicylic acid (SA) and methyl jasmonate (Meja) could induce the up-regulation of CsSAMT-1 and the contents of methyl salicylate (MeSA) in the leaf veins of disease-tolerant grapefruit were 11.85 and 18.18 ng g ~ (-1), respectively, which were significantly higher than those of Jincheng (0.82 and 0.01 ng g ~ (-1) HLB. The contents of SA and MeSA in Jincheng orange were obviously increased, while the contents of SA in sour grapefruit were obviously decreased, and the contents of MeSA were basically maintained at the same level. The results showed that SAMT-1 may enhance the resistance to citrus yellow dragon disease by regulating the transformation of SA and me SA signaling molecules.
【作者单位】：西南大学/中国农业科学院柑桔研究所国家柑桔工程技术研究中心国家柑桔品种改良中心;
【基金】：重庆市基础科学与前沿技术研究项目(cstc2017jcyj BX0020) 国家现代农业产业技术体系建设专项资金项目(CARS-27) 重庆市社会事业与民生保障科技创新专项项目(cstc2016shms-ztzx80001)
【分类号】：S436.66

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