不同剂量氯胺酮对体外循环缺血再灌注心肌铜锌超氧化物歧化酶基因表达的影响
发布时间：2018-07-13 11:15
【摘要】：目的探讨体外循环(CPB)手术中氯胺酮(KET)在缺血再灌注心肌对铜锌超氧化物歧化酶(CuZn SOD)基因表达的影响及其作用机制。方法选取2006年3-9月在甘肃省人民医院心外科行先天性心脏病修补手术的30例患者为研究对象,随机分为A、B、C组(各10例),B组和C组分别静脉滴注1 mg/kg和0.5 mg/kg的KET,A组给予同等量0.9%氯化钠溶液干预。于主动脉阻断前、主动脉阻断后1h、主动脉开放后30 min从右心耳分别提取心肌组织,提取的心肌组织总核糖核酸(RNA)通过荧光定量反转录-聚合酶链反应(RT-PCR),以β肌动蛋白(β-actin)为内参对照Cu-Zn SOD mRNA的表达水平进行检测。结果 3组在主动脉阻断前心肌组织CuZn SOD mRNA比较,差异无统计学意义(P0.05);A组在主动脉阻断后1 h及主动脉开放后30 min心肌组织CuZn SOD mRNA低于B组和C组,差异有统计学意义(P0.05);B组和C组在主动脉阻断后1 h及主动脉开放后30min心肌组织Cu-Zn SOD mRNA比较,差异无统计学意义(P0.05)。结论 KET可提高缺血再灌注心肌组织中Cu-Zn SOD mRNA含量,抑制大量氧自由基生成,从而发挥抗氧化、抗炎作用,进而保护心肌。
[Abstract]:Objective to investigate the effect of ketamine (KET) on the expression of copper and zinc superoxide dismutase (CuZn SOD) during cardiopulmonary bypass (CPB) and its mechanism. Methods Thirty patients with congenital heart disease were selected from March to September, 2006, who underwent congenital heart repair surgery in the Department of Cardiac surgery, people's Hospital of Gansu Province. The rats were randomly divided into two groups: group B (n = 10) and group C (n = 10) treated with the same amount of sodium chloride solution for 1 mg/kg and 0.5 mg/kg respectively. Myocardial tissue was extracted from the right atrial appendage 30 min after aortic occlusion and 1 hour after aortic occlusion. Total ribonucleic acid (RNA) was extracted from myocardial tissue and the expression level of Cu-Zn SOD mRNA was detected by fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR) and 尾 -actin as internal control. Results there was no significant difference in CuZn SOD mRNA between the three groups before aortic occlusion (P0.05). The CuZn SOD mRNA in myocardial tissue in group A was lower than that in group B and C at 1 h after aortic occlusion and 30 min after aortic opening. The difference was statistically significant (P0.05). There was no significant difference in Cu-Zn SOD mRNA between group B and group C at 1 hour after aortic occlusion and in myocardial tissue of 30min after aortic opening (P0.05). Conclusion KET can increase the content of Cu-Zn SOD mRNA and inhibit the production of oxygen free radicals in ischemic reperfusion myocardium, thus exerting anti-oxidation and anti-inflammatory effects, and then protecting myocardium.
【作者单位】： 甘肃省人民医院心内科;甘肃医学院微生物教研室;甘肃省人民医院产科;甘肃省人民医院心外科;
【分类号】：R614
本文编号：2119164
[Abstract]:Objective to investigate the effect of ketamine (KET) on the expression of copper and zinc superoxide dismutase (CuZn SOD) during cardiopulmonary bypass (CPB) and its mechanism. Methods Thirty patients with congenital heart disease were selected from March to September, 2006, who underwent congenital heart repair surgery in the Department of Cardiac surgery, people's Hospital of Gansu Province. The rats were randomly divided into two groups: group B (n = 10) and group C (n = 10) treated with the same amount of sodium chloride solution for 1 mg/kg and 0.5 mg/kg respectively. Myocardial tissue was extracted from the right atrial appendage 30 min after aortic occlusion and 1 hour after aortic occlusion. Total ribonucleic acid (RNA) was extracted from myocardial tissue and the expression level of Cu-Zn SOD mRNA was detected by fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR) and 尾 -actin as internal control. Results there was no significant difference in CuZn SOD mRNA between the three groups before aortic occlusion (P0.05). The CuZn SOD mRNA in myocardial tissue in group A was lower than that in group B and C at 1 h after aortic occlusion and 30 min after aortic opening. The difference was statistically significant (P0.05). There was no significant difference in Cu-Zn SOD mRNA between group B and group C at 1 hour after aortic occlusion and in myocardial tissue of 30min after aortic opening (P0.05). Conclusion KET can increase the content of Cu-Zn SOD mRNA and inhibit the production of oxygen free radicals in ischemic reperfusion myocardium, thus exerting anti-oxidation and anti-inflammatory effects, and then protecting myocardium.
【作者单位】： 甘肃省人民医院心内科;甘肃医学院微生物教研室;甘肃省人民医院产科;甘肃省人民医院心外科;
【分类号】：R614
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