2014-2016年柳州市甲型H3流感病毒血凝素抗原表位基因变异和进化分析
发布时间：2018-07-15 22:01
【摘要】：目的通过分析近年柳州市甲型H3流感病毒HA抗原表位基因突变情况和进化分析,了解其分子流行及变异变迁规律。方法采集2014-2016年柳州市哨点医院以及流感突发疫情病例的呼吸道标本。提取标本核酸后用实时荧光PCR检测,对甲型H3阳性标本扩增血凝素HA基因片段、测序和进行基因变异分析。结果在2014-2016年间总共检测5 164例病例,503例为甲型H3流感病毒阳性。分析2014-2016年柳州市获得的41个甲型H3流感病毒HA基因全长序列的系统进化树发现:2014-2016年间甲型H3流感病毒共引起了3个主要的流感流行峰,且分别属于两个不同的基因簇A/Switzerland/9715293/2013类似株(SW13)为Group1和A/Hong Kong/4801/2014类似株(HK14)Group 2。比较3个流行峰发现血凝素蛋白氨基酸序列出现点突变,主要分布在抗原表位和受体结合位点。结论2014-2016年柳州市流行的甲型H3流感病毒,与当年WHO推荐疫苗株比较出现滞后现象,出现两次明显的抗原漂变,可能是导致柳州市近年来甲型H3流感流行的原因。
[Abstract]:Objective to analyze the mutation and evolution of HA epitope gene of influenza A (H3) virus in Liuzhou City in recent years and to understand its molecular prevalence and variation. Methods Respiratory tract specimens from sentinel hospital and influenza outbreak cases were collected from 2014-2016. The HA gene fragment of hemagglutinin was amplified and sequenced and analyzed by real-time fluorescence PCR. Results A total of 5 164 cases were detected in 2014-2016. 503 cases were positive for influenza A H 3 virus. The phylogenetic tree of 41 full-length HA gene sequences of influenza A / H3 virus obtained from Liuzhou City from 2014-2016 shows that the influenza A / H3 virus caused three major influenza epidemic peaks between 2014 and 2016. And belong to two different clusters of gene clusters, A / R / 9715293 / 2013 (SW13), respectively, are Group1 and A / Hong Kong.4801 / 2014 (HK14) Group 2. The amino acid sequence of hemagglutinin protein was found to have point mutations, mainly in antigen epitopes and receptor binding sites. Conclusion compared with the vaccine strains recommended by WHO, the influenza A H3 virus in Liuzhou from 2014-2016 to 2016 is lagging behind, and two obvious antigen bleaching appears, which may be the cause of influenza A H3 epidemic in Liuzhou in recent years.
【作者单位】： 柳州市疾病预防控制中心微生物检验科;
【基金】：广西卫生厅自筹课题(E2011265)
【分类号】：R511.7
，
本文编号：2125511
[Abstract]:Objective to analyze the mutation and evolution of HA epitope gene of influenza A (H3) virus in Liuzhou City in recent years and to understand its molecular prevalence and variation. Methods Respiratory tract specimens from sentinel hospital and influenza outbreak cases were collected from 2014-2016. The HA gene fragment of hemagglutinin was amplified and sequenced and analyzed by real-time fluorescence PCR. Results A total of 5 164 cases were detected in 2014-2016. 503 cases were positive for influenza A H 3 virus. The phylogenetic tree of 41 full-length HA gene sequences of influenza A / H3 virus obtained from Liuzhou City from 2014-2016 shows that the influenza A / H3 virus caused three major influenza epidemic peaks between 2014 and 2016. And belong to two different clusters of gene clusters, A / R / 9715293 / 2013 (SW13), respectively, are Group1 and A / Hong Kong.4801 / 2014 (HK14) Group 2. The amino acid sequence of hemagglutinin protein was found to have point mutations, mainly in antigen epitopes and receptor binding sites. Conclusion compared with the vaccine strains recommended by WHO, the influenza A H3 virus in Liuzhou from 2014-2016 to 2016 is lagging behind, and two obvious antigen bleaching appears, which may be the cause of influenza A H3 epidemic in Liuzhou in recent years.
【作者单位】： 柳州市疾病预防控制中心微生物检验科;
【基金】：广西卫生厅自筹课题(E2011265)
【分类号】：R511.7
，
本文编号：2125511
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