KIR 3DL3基因测序分型中模棱两可结果的鉴定方法
发布时间：2018-07-16 14:07
【摘要】：目的探讨KIR3DL3基因测序分型中出现的模棱两可结果的鉴定方法。方法对南方汉族614例白血病患者的KIR3DL3基因的全部编码区进行测序分型,统计KIR3DL3模棱两可等位基因结果的种类及比例,针对每种模棱两可结果中等位基因编码区序列碱基的差异,设计组特异性PCR引物,进行组特异性KIR3DL3基因PCR扩增和再测序,鉴定KIR3DL3的基因型。结果共检出了7种新的KIR3DL3模棱两可的结果,其中3DL3*(00301,01002/00902,028)检出了7例,占1.14%(7/614);3DL3*(00102,01501/00601,00901/02102,023)、3DL3*(00402,01002/00802,028)、3DL3*(00402,01001/00801,028)分别检出了6例,均占0.98%(6/614);3DL3*(00301,01001/00901,028)、3DL3*(00301,00801/00402,00901)、3DL3*(00301,00802/00402,00902)分别检出了3例,均占0.49%(3/614)。这7种模棱两可组合分别采用组特异性PCR引物均能准确的鉴定出KIR3DL3基因型,其中3DL3*(00102,01501/00601,00901/02102,023)、3DL3*(00402,01001/00801,028)和3DL3*(00301,00802/00402,00902)的模棱两可组合样本均鉴定出1种基因型,其余的模棱两可组合均检出了2种不同的基因型。结论本文为鉴定KIR3DL3模棱两可的结果建立了可靠的鉴定方法,具有良好的实用价值。
[Abstract]:Objective to investigate the identification of ambiguous results in KIR3 DL3 genotyping. Methods all coding regions of KIR3DL3 gene in 614 patients with leukemia in southern Han nationality were sequenced, and the types and proportions of the results of KIR3DL3 ambiguous alleles were analyzed. The gene of KIR3DL3 gene was amplified by PCR and sequenced. The genotypes of KIR3DL3 gene were identified. 缁撴灉鍏辨鍑轰簡7绉嶆柊鐨凨IR3DL3妯℃１涓ゅ彲鐨勭粨鏋，
本文编号：2126630
[Abstract]:Objective to investigate the identification of ambiguous results in KIR3 DL3 genotyping. Methods all coding regions of KIR3DL3 gene in 614 patients with leukemia in southern Han nationality were sequenced, and the types and proportions of the results of KIR3DL3 ambiguous alleles were analyzed. The gene of KIR3DL3 gene was amplified by PCR and sequenced. The genotypes of KIR3DL3 gene were identified. 缁撴灉鍏辨鍑轰簡7绉嶆柊鐨凨IR3DL3妯℃１涓ゅ彲鐨勭粨鏋，
本文编号：2126630
本文链接：https://www.wllwen.com/kejilunwen/jiyingongcheng/2126630.html
最近更新
教材专著
