草地早熟禾叶绿素酶1基因PpCHL1的克隆和表达分析
发布时间：2018-07-16 21:11
【摘要】：利用RACE技术首次从草地早熟禾中克隆出叶绿素酶1基因(PpCHL1)的全长cDNA序列,提交到GenBank,登录号为KU145126;其开放阅读框为951bp,编码361个氨基酸,等电点6.11,平均亲水指数0.084,属于疏水性酸性蛋白。高级结构分析表明,其具有脂肪酶家族的保守域及水解酶特异的功能活性位点。利用实时荧光定量PCR分析其在草地早熟禾根、茎和叶及三种植物生长调节剂脱落酸(ABA)、水杨酸(SA)和茉莉酸甲酯(MeJA)处理后的表达情况,结果显示PpCHL1基因在草地早熟禾不同组织的表达情况存在明显的组织差异性,叶中的表达量最高且受ABA、SA和MeJA诱导。
[Abstract]:The full-length cDNA sequence of chlorophyll enzyme 1 gene (PpCHL1) was cloned from Kentucky bluegrass by race technique for the first time. The cDNA sequence was submitted to GenBank, the accession number was KU145126, and the open reading frame was 951 BP, encoding 361 amino acids. Isoelectric point 6.11, average hydrophilic index 0.084, belong to hydrophobic acid protein. The advanced structure analysis showed that it had the conserved domain of lipase family and the functional activity site of hydrolase. The expression of POA in roots, stems and leaves of Kentucky bluegrass and three plant growth regulators, abscisic acid (ABA), salicylic acid (SA) and methyl jasmonate (Meja), was analyzed by real-time fluorescence quantitative PCR. The results showed that the expression of PpCHL1 gene in different tissues of Kentucky bluegrass was significantly different, and the highest expression level of PpCHL1 gene was found in the leaves, which was induced by ABANASA and Meja.
【作者单位】： 北京林业大学草坪研究所;
【基金】：国家自然科学基金项目“空间诱变草地早熟禾矮化突变体矮化机理研究”(31302016)
【分类号】：S688.4
[Abstract]:The full-length cDNA sequence of chlorophyll enzyme 1 gene (PpCHL1) was cloned from Kentucky bluegrass by race technique for the first time. The cDNA sequence was submitted to GenBank, the accession number was KU145126, and the open reading frame was 951 BP, encoding 361 amino acids. Isoelectric point 6.11, average hydrophilic index 0.084, belong to hydrophobic acid protein. The advanced structure analysis showed that it had the conserved domain of lipase family and the functional activity site of hydrolase. The expression of POA in roots, stems and leaves of Kentucky bluegrass and three plant growth regulators, abscisic acid (ABA), salicylic acid (SA) and methyl jasmonate (Meja), was analyzed by real-time fluorescence quantitative PCR. The results showed that the expression of PpCHL1 gene in different tissues of Kentucky bluegrass was significantly different, and the highest expression level of PpCHL1 gene was found in the leaves, which was induced by ABANASA and Meja.
【作者单位】： 北京林业大学草坪研究所;
【基金】：国家自然科学基金项目“空间诱变草地早熟禾矮化突变体矮化机理研究”(31302016)
【分类号】：S688.4
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