规律运动对心脏特异性Ca-α1D基因沉默老龄果蝇心脏节律和收缩功能的影响
发布时间：2018-07-17 02:34
【摘要】：目的:研究青年期开始规律运动对心脏Ca-α1D基因沉默老龄果蝇心脏功能、运动能力和生命周期的影响,探讨Ca-α1D基因在心脏衰老及运动抗心脏衰老中的作用。方法:基因型为w1118;P{GD1737}v51491的处女蝇分别与W1118品系、Hand-GAL4品系雄蝇杂交,获得F1代处女蝇分为安静组、干扰组、运动组、运动干扰组,每组400只,饲养至2周龄开始运动,2小时/天,5天/周,训练持续时间为4周。采用qRT-PCR测定心肌Ca-α1D基因mRNA表达量,M-mode心动图检测评价心脏功能,测量攀爬指数评定运动能力,统计生命周期反映寿命情况。结果:(1)干扰组心肌Ca-α1D的相对表达量较对照组约下降50%(P0.01)。(2)与对照组相比,干扰组心动周期(HP)增加(P0.01),收缩间期(SI)上升(P0.05)。纤维性震颤(FL)、心动徐缓发生率增加(P0.01)。舒张直径(DD)、收缩直径(SD)增加(P0.01),射血分数(FS)降低(P0.05)。与对照组相比,运动组HP、DI上升(P0.05),心动徐缓发生率增加(P0.01)。与对照组相比,运动干扰组HP、心动徐缓发生率增加(P0.01)。DD、SD增加(P0.01),而FS变化不具有显著性差异(P0.05)。与干扰组相比,运动干扰组心动徐缓发生率减少(P0.01)。DD增加(P0.05)。基因沉默联合耐力运动对心动徐缓发生率的结果有协同作用(P0.05)。其中基因是对HP、DD、SD、FS、FL结果产生作用的主效应,运动是对DI结果产生作用的主效应。(3)与对照组相比,运动组攀爬能力增强(P0.01)、干扰组攀爬能力降低(P0.05);基因沉默联合耐力运动对攀爬能力具有协同作用(P0.05)。(4)运动组平均寿命高于对照组(P0.01),运动干扰组高于干扰组(P0.01),干扰组平均寿命低于对照组(P0.01)。结论:规律运动的干预能够维持心脏正常节律、提高运动能力和增进生命周期有一定作用。心脏特异性Ca-α1D基因沉默是导致该果蝇心脏收缩功能减退、节律紊乱以及寿命缩减的主要原因。运动能够有效改善因Ca-α1D基因敲减引起的心动过缓、提高运动能力,但是对心脏收缩能力的改善作用不显著,其原因是否与老龄果蝇自身的机能水平衰退有关,值得进一步研究。
[Abstract]:Objective: To study the effect of Ca- alpha 1D gene on cardiac function, exercise ability and life cycle of aged Drosophila melanogaster, and to explore the role of Ca- alpha 1D gene in cardiac senescence and anti cardiac senescence. Methods: gene type is w1118; P{GD1737}v51491 virginity flies respectively with W1118 strain and Hand-GAL4 strain male flies, respectively. The F1 virginity flies were divided into the quiet group, the interference group, the exercise group and the exercise interference group, 400 rats in each group, and were fed to 2 weeks of age to start the movement, 2 hours / day, 5 days / week, and the training duration was 4 weeks. The mRNA expression of Ca- alpha 1D gene was measured by qRT-PCR, and the cardiac function was evaluated by M-mode cardiogram, and the climbing index was used to assess the ability of exercise and to measure climbing index to assess exercise ability and to measure the ability of climbing index. Life cycle reflected life expectancy. Results: (1) the relative expression of Ca- alpha 1D in the myocardium of the interference group decreased by 50% (P0.01). (2) compared to the control group, the cardiac cycle (HP) increased (P0.01), the Systolic Interval (SI) increased (P0.05), fibrous tremor (FL), the rate of bradycardia increased (P0.01). The diastolic diameter (DD), the contraction diameter (SD) increased. Plus (P0.01), ejection fraction (FS) decreased (P0.05). Compared with the control group, the exercise group was HP, DI (P0.05), and the incidence of bradycardia increased (P0.01). Compared with the control group, the incidence of HP in the exercise group was increased (P0.01).DD, SD increased (P0.01), but there was no significant difference between the exercise group and the disturbance group. The incidence of P0.01.DD increased (P0.05). Gene silencing combined with endurance exercise had synergistic effects on the incidence of bradycardia (P0.05). Gene was the main effect on HP, DD, SD, FS, and FL, and movement was the main effect on the results of DI. (3) compared with the control group, the climbing ability of the exercise group was enhanced (P0.01), dry The climbing ability of the group was reduced (P0.05); gene silencing combined with endurance exercise had synergistic effect on climbing ability (P0.05). (4) the average life span of the exercise group was higher than that of the control group (P0.01), the exercise interference group was higher than the interference group (P0.01), and the average life span of the interference group was lower than that of the control group (P0.01). Conclusion: the intervention of regular exercise can maintain the normal rhythm of the heart, and improve the normal rhythm of the heart. The heart specific Ca- alpha 1D gene silencing is the main cause of the Drosophila's systolic dysfunction, rhythmic disorder, and life expectancy. Exercise can effectively improve the bradycardia caused by Ca- alpha 1D knockout and improve the movement ability, but the improvement of cardiac contractility is improved. It is not obvious whether the reason is related to the decline of the functional level of the Drosophila melanogaster.
【作者单位】： 湖南师范大学体育学院;
【基金】：湖南省自然科学基金项目(2015JJ2102)
【分类号】：G804.2
本文编号：2128630
[Abstract]:Objective: To study the effect of Ca- alpha 1D gene on cardiac function, exercise ability and life cycle of aged Drosophila melanogaster, and to explore the role of Ca- alpha 1D gene in cardiac senescence and anti cardiac senescence. Methods: gene type is w1118; P{GD1737}v51491 virginity flies respectively with W1118 strain and Hand-GAL4 strain male flies, respectively. The F1 virginity flies were divided into the quiet group, the interference group, the exercise group and the exercise interference group, 400 rats in each group, and were fed to 2 weeks of age to start the movement, 2 hours / day, 5 days / week, and the training duration was 4 weeks. The mRNA expression of Ca- alpha 1D gene was measured by qRT-PCR, and the cardiac function was evaluated by M-mode cardiogram, and the climbing index was used to assess the ability of exercise and to measure climbing index to assess exercise ability and to measure the ability of climbing index. Life cycle reflected life expectancy. Results: (1) the relative expression of Ca- alpha 1D in the myocardium of the interference group decreased by 50% (P0.01). (2) compared to the control group, the cardiac cycle (HP) increased (P0.01), the Systolic Interval (SI) increased (P0.05), fibrous tremor (FL), the rate of bradycardia increased (P0.01). The diastolic diameter (DD), the contraction diameter (SD) increased. Plus (P0.01), ejection fraction (FS) decreased (P0.05). Compared with the control group, the exercise group was HP, DI (P0.05), and the incidence of bradycardia increased (P0.01). Compared with the control group, the incidence of HP in the exercise group was increased (P0.01).DD, SD increased (P0.01), but there was no significant difference between the exercise group and the disturbance group. The incidence of P0.01.DD increased (P0.05). Gene silencing combined with endurance exercise had synergistic effects on the incidence of bradycardia (P0.05). Gene was the main effect on HP, DD, SD, FS, and FL, and movement was the main effect on the results of DI. (3) compared with the control group, the climbing ability of the exercise group was enhanced (P0.01), dry The climbing ability of the group was reduced (P0.05); gene silencing combined with endurance exercise had synergistic effect on climbing ability (P0.05). (4) the average life span of the exercise group was higher than that of the control group (P0.01), the exercise interference group was higher than the interference group (P0.01), and the average life span of the interference group was lower than that of the control group (P0.01). Conclusion: the intervention of regular exercise can maintain the normal rhythm of the heart, and improve the normal rhythm of the heart. The heart specific Ca- alpha 1D gene silencing is the main cause of the Drosophila's systolic dysfunction, rhythmic disorder, and life expectancy. Exercise can effectively improve the bradycardia caused by Ca- alpha 1D knockout and improve the movement ability, but the improvement of cardiac contractility is improved. It is not obvious whether the reason is related to the decline of the functional level of the Drosophila melanogaster.
【作者单位】： 湖南师范大学体育学院;
【基金】：湖南省自然科学基金项目(2015JJ2102)
【分类号】：G804.2
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