陕西省部分地区PRRSV流行毒株ORF3和ORF5基因的遗传变异分析

发布时间：2018-07-21 15:02

【摘要】：为了解陕西省猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)流行毒株的遗传和变异情况,对9份疑似感染PRRSV的病料用RT-PCR方法分别扩增ORF3和ORF5基因,并进行测序和序列分析。测序结果表明,ORF3基因在254-490和646-737位置之间发生不连续碱基突变;与GenBank已发表的国外毒株ORF3基因组比较,同源性为89.2%~94.8%,与国内发表的ORF3基因比较,同源性为88.8%~99.0%,其中与2009年陕西分离株(HQ843181.1)同源性为94.9%~98.7%,与2010年陕西株(KJ855518.1)同源性为94.9%~98.6%。ORF5基因突变主要在508-600碱基位置之间,与GenBank上已发表的国外毒株ORF5基因组比较,同源性为90.1%~92.1%,与国内发表的ORF5基因比较,同源性为89.6%~99.2%,其中与2009年陕西分离株(HQ843181.1)同源性为95.2%~98.4%,与2010年陕西株(KJ855518.1)同源性为95.2%~98.7%。ORF3和ORF5基因进化树中Wn-2、Ak-3和Tc-4毒株在同一个进化支上,之间亲缘关系近,Xy-1和Hz-5毒株分别在不同的进化分支上,与其他毒株遗传距离较远。陕西省PRRSV流行毒株的ORF5和ORF3基因组与国内PRRSV分离株相比较均有一定变异,且亲缘进化不完全相同。
[Abstract]:In order to understand the heredity and variation of the Porcine reproductive and respiratory syndrome virus (PRRSV) virus (PRRSV) virus in Shaanxi Province, the ORF3 and ORF5 genes were amplified by RT-PCR method for 9 suspected PRRSV infected materials, and the sequence and sequence analysis were carried out. The sequencing results showed that the ORF3 genes were 254-490 and 6. The discontinuous base mutation occurred between the 46-737 positions; compared with the published ORF3 genome of GenBank, the homology was 89.2%~94.8%, and the homology was 88.8%~99.0% compared with the ORF3 gene published in China. The homology of the Shaanxi isolate (HQ843181.1) in 2009 was 94.9%~98.7%, and the homology of the Shaanxi strain (KJ855518.1) in 2010 was 94.9%~ The 98.6%.ORF5 gene mutation is mainly between the 508-600 base sites, and the homology is 90.1%~92.1% compared with the published foreign virus ORF5 genome on GenBank. Compared with the ORF5 gene published in China, the homology is 89.6%~99.2%. The homology of the Shaanxi isolate (HQ843181.1) in 2009 is 95.2%~98.4%, and the same as that of the 2010 Shaanxi strain (KJ855518.1). Wn-2, Ak-3 and Tc-4 strains in the phylogenetic tree of 95.2%~98.7%.ORF3 and ORF5 are closely related to the same evolutionary branch. Xy-1 and Hz-5 strains are on different evolutionary branches and are far away from other strains. The ORF5 and ORF3 base of PRRSV epidemic strains in Shaanxi province are compared with those of PRRSV isolates in China. Variation and phylogenetic evolution are not exactly the same.
【作者单位】：西北农林科技大学动物医学院;
【基金】：陕西省农业科技创新与攻关项目(2016NY-095) 西北农林科技大学基本科研业务费项目(Z109021427)
【分类号】：S852.651

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