基于转录组学技术探究中华绒螯蟹胚胎发育期间的基因和通路表达特征
[Abstract]:As one of the largest groups of invertebrates, crustaceans have rich biological diversity and complex life history. As an important developmental period of crustaceans, the embryonic stage has undergone a series of changes in the regulation of gene development and expression patterns. The study of subbiological mechanism is not sufficient, especially in the animal of Eriocheir sinensis. In this paper, the embryo of Eriocheir sinensis in the Changjiang River system was studied in El, E2, E3, E4, E5, E6, E7 and Z1. A number of important functional genes related to growth and development are obtained, and the molecular basis of important physiological phenomena in this development process is preliminarily analyzed. The main research results of this paper are as follows: 1. based on the Illumina Hiseq2000 sequencing platform, a total of 108338422 original double end sequence reading segments were obtained by data preprocessing and Trinity software spelling. 60985 non redundant genes were obtained, with an average length of 614bp, and 30488 of them were annotated in the Nr, Swiss-Prot, GO, COG, KEGG and other databases. By statistical analysis of the transcriptional data of the 8 embryonic development period, we obtained the gene expression profiles at the stage of embryo development throughout the river crab. At the same time, we used the hierarchy. 8 embryos samples were divided into 4 periods by clustering, principal component analysis and correlation analysis, which corresponded to the early stage (El-E2), middle stage (E3-E5), post (E6-E7) and early hatchle (Z1) period of the embryo development of the river crab. There are 1123 different genes in the early and middle stages, which are enriched in the cell differentiation and development related processes, the metabolism and transport of organic matter and the endogenous stimulus response. From metaphase to late development, 3422 differentially expressed genes are obtained. They are degraded and metabolizing exogenous organisms, lipid metabolism, amino acid metabolism, and modulation. The process, the occurrence and development of muscle, the process of muscle protein and myofibrillar assembly are enriched, and 1729 different genes are obtained from the late embryonic development to the early hatchlings. They are mainly enriched in the process of translation, metabolism, myofibrillar assembly and muscle development, the process of organic matter decomposition, transport and protein stability. We identified 3303 genes that were expressed continuously throughout the development stage of the embryo, and their expression changes in the whole development stage were more intense, showing a certain spatio-temporal expression characteristic.2. will obtain the data set of the crab embryo development transcriptional group and the river crab sketching genome, the EST data of the river crab, and the three species of the orodrosophila melanogaster and the silkworm. Compared with the genomic data of the freshwater horns flea, the sequence information and characteristics of the data of the river crab transcriptional group were preliminarily analyzed. In order to study the sequence structure of the related functional genes in the embryonic stage of the river crab and the selective shear events,.3. based on the data of the group, we identified the related genes (including 794 transcripts), and the metabolic related related genes. Gene data set (including 1382 transcripts) and immune related genes (including 662 transcripts), which identified 10 parent effect genes: aristaless, Bicaudal, Goosecoid, Homeobox protein OTX2-A, nanos, polehole, pumilio, Staufen, Tudor and vasa; the interval gene was 6, respectively. Al, crocodile, empty spiracles, giant, knirps-like and Kr u ppel genes. The control genes (pair-rule genes) are 8, respectively. Frizzled, fused, gooseberry, Invected, lines, pangolin, patched, roadkill, smoothened, Wnt, and Wntless genes. Hedgehog, JAK-STAT pathway, MAPK, TGF- beta, Toll like receptor, NOD like receptor, Wnt and so on regulate the key signaling pathways in the regulation of embryonic formation and development, and analyze the expression of genes in the pathway in different periods. In addition, we are based on the table of lipid metabolism related genes and tables in the embryo development of Eriocheir sinensis. Information has been drawn to map the metabolic network of embryonic development. These visual annotation information provide a favorable resource for understanding the metabolic pathways of embryonic development.
【学位授予单位】:上海海洋大学
【学位级别】:硕士
【学位授予年份】:2017
【分类号】:S917.4
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