仿刺参体壁再生过程中两个核糖体蛋白基因的表达差异
发布时间：2018-07-25 12:43
【摘要】：本研究首次从仿刺参(Apostichopus japonicus)体壁中克隆得到核糖体蛋白L30(ribosomal protein L30,RPL30)的c DNA全长序列(Gen Bank:JQ770165),该序列包括56 bp的5′-UTR,162 bp的3′-UTR和339b p的开放阅读框,共编码112个氨基酸;Blast比对分析结果显示该序列的核苷酸序列以及推导的氨基酸序列与其他物种的同源性均在75%以上;RPL30基因在仿刺参各组织中均有表达,其中肠、体腔细胞、纵肌、体壁中表达量分别为呼吸树的7.24倍(P0.01)、4.47倍(P0.01)、3.12倍(P0.05)、1.35倍(P0.05);仿刺参体壁再生不同阶段核糖体蛋白基因RPL30和RPL17的表达存在差异,体壁再生第7 d时RPL30基因的表达出现峰值,为对照组的2.13倍(P0.05),其余天数的相对表达量均低于对照组,其中第1、5、6d的表达量与对照组差异均显著(P0.05);RPL17基因在再生第2、5d分别出现峰值,分别为对照组的7.47倍和5.60倍(P0.05),其余各天的表达量与对照组差异不显著(P0.05)。研究结果表明核糖体蛋白基因除构成核糖体参与蛋白质合成外,还有着各自的核糖体外功能。本研究结果将为进一步研究仿刺参蛋白质合成、再生及多种生理活动的调控机制奠定基础。
[Abstract]:In this study, the full-length DNA sequence (Gen Bank:JQ770165) of ribosomal protein L30 (ribosomal protein L30RPL30 was first cloned from the body wall of (Apostichopus japonicus). The sequence consists of 56 BP 3'-UTR and 339bp open reading frame. The results of cocoding 112 amino acid blast analysis showed that the nucleotide sequence of the sequence and the deduced amino acid sequence had more than 75% homology with other species. The RPL30 gene was expressed in all tissues, including intestinal and somatic cells. The expression of ribosomal protein gene RPL30 and RPL17 in longitudinal muscle and body wall were 7.24 times (P0.01) 4.47 times (P0.01) 3.12 times (P0.05) and 1.35 times (P0.05) of respiratory tree, respectively, the expression of ribosomal protein gene RPL30 and RPL17 were different in different stages of body wall regeneration, and the peak expression of RPL30 gene appeared at the 7th day of wall regeneration. The relative expression of RPL17 gene in the control group was 2.13 times of that in the control group (P0.05), and the relative expression of RPL17 gene in the other days was lower than that in the control group. There were significant differences in the expression of RPL17 gene between the control group and the control group on the 6th day (P0.05) and the peak value of RPL17 gene on the 2nd day of regeneration. It was 7.47 times and 5.60 times of the control group respectively (P0.05). The expression of the other days was not significantly different from that of the control group (P0.05). The results showed that ribosomal protein genes had their own ribosomal functions in addition to ribosomes involved in protein synthesis. The results of this study will lay a foundation for the further study of protein synthesis, regeneration and regulation mechanism of various physiological activities of Acanthopsis japonicus.
【作者单位】： 大连海洋大学辽宁省海洋生物资源与生境修复重点实验室;大连海洋大学农业部北方海水增养殖重点实验室;
【基金】：国家自然科学基金(30371099) 辽宁省教育厅创新团队(2007T015);辽宁省教育厅实验室专项(2008S064)
【分类号】：S917.4
[Abstract]:In this study, the full-length DNA sequence (Gen Bank:JQ770165) of ribosomal protein L30 (ribosomal protein L30RPL30 was first cloned from the body wall of (Apostichopus japonicus). The sequence consists of 56 BP 3'-UTR and 339bp open reading frame. The results of cocoding 112 amino acid blast analysis showed that the nucleotide sequence of the sequence and the deduced amino acid sequence had more than 75% homology with other species. The RPL30 gene was expressed in all tissues, including intestinal and somatic cells. The expression of ribosomal protein gene RPL30 and RPL17 in longitudinal muscle and body wall were 7.24 times (P0.01) 4.47 times (P0.01) 3.12 times (P0.05) and 1.35 times (P0.05) of respiratory tree, respectively, the expression of ribosomal protein gene RPL30 and RPL17 were different in different stages of body wall regeneration, and the peak expression of RPL30 gene appeared at the 7th day of wall regeneration. The relative expression of RPL17 gene in the control group was 2.13 times of that in the control group (P0.05), and the relative expression of RPL17 gene in the other days was lower than that in the control group. There were significant differences in the expression of RPL17 gene between the control group and the control group on the 6th day (P0.05) and the peak value of RPL17 gene on the 2nd day of regeneration. It was 7.47 times and 5.60 times of the control group respectively (P0.05). The expression of the other days was not significantly different from that of the control group (P0.05). The results showed that ribosomal protein genes had their own ribosomal functions in addition to ribosomes involved in protein synthesis. The results of this study will lay a foundation for the further study of protein synthesis, regeneration and regulation mechanism of various physiological activities of Acanthopsis japonicus.
【作者单位】： 大连海洋大学辽宁省海洋生物资源与生境修复重点实验室;大连海洋大学农业部北方海水增养殖重点实验室;
【基金】：国家自然科学基金(30371099) 辽宁省教育厅创新团队(2007T015);辽宁省教育厅实验室专项(2008S064)
【分类号】：S917.4
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