长江刀鲚mor-4k13基因的分离鉴定及表达分析

发布时间：2018-07-25 15:04

【摘要】：分布于长江的刀鲚(Coilia nasus)具有洄游和定居两个生态型,生殖洄游是区别两者的主要表征。为了探索嗅觉受体(OR)基因是否参与了刀鲚的生殖洄游过程,本文采用RACE技术从洄游型刀鲚中获得了mor-4k13基因,该基因全长1 098 bp,编码区长963 bp,单外显子结构,可编码320个氨基酸。预测表明,mor-4k13基因编码的蛋白质,为7个疏水性的α-螺旋跨膜结构,属G-蛋白偶联受体,有胆固醇和油酸两个配体。MOR-4K13蛋白与已报道的其他鱼类OR蛋白的同源性在40%~68%之间,其中,与近缘种大西洋鲱(Clupea harengus)嗅觉受体蛋白同源性高达68%。采用Real-time PCR方法对10个组织或器官所作的荧光定量分析显示,mor-4k13基因在定居型刀鲚嗅囊和性腺中高表达,在肌肉、眼球、胃壁、肝和鳃中低表达,心肌中几乎不表达。mor-4k13基因在洄游型刀鲚嗅囊中的表达量总体高于定居型,且洄游型雄性刀鲚嗅囊中此基因的表达量约是其雌性嗅囊中的3倍。这表明mor-4k13基因不仅与嗅觉功能和性腺发育相关,也可能与生殖洄游习性相关,不同性别的个体间也存在着嗅觉能力的差异。
[Abstract]:There are two ecotypes of Coilia ecotype, (Coilia nasus), which distributes in the Yangtze River, and reproductive migration is the main characteristic of distinguishing the two ecotypes. In order to find out whether the olfactory receptor (OR) gene is involved in the reproductive migration of Coilia ecystus, the mor-4k13 gene was obtained from Coilia ectenes by RACE technique. The gene is 1 098 BP in length and encodes 963 BP in length, with a single exon structure and encoding 320 amino acids. It is predicted that the protein encoded by the gene is seven hydrophobic 伪 -helix transmembrane structures, belonging to G-protein-coupled receptors. The homology of OR proteins of cholesterol and oleic acid ligands .MOR-4K13 with other reported fish is between 40% and 68%. The protein homology of olfactory receptor protein of (Clupea harengus) in Atlantic herring is 68%. Fluorescence quantitative analysis of 10 tissues or organs by Real-time PCR method showed that mor-4k13 gene was highly expressed in olfactory sac and gonad of Coilia ectenes, and was low expressed in muscle, eyeball, stomach wall, liver and Gill. The expression of .mor-4k13 gene in the migratory Coilia ectenes was generally higher than that in the sedentary type, and the expression of this gene in the migratory male Coilia ectenes was about three times higher than that in the female olfactory sac. This suggests that mor-4k13 gene is not only related to olfactory function and gonad development, but also related to reproductive migration, and there are differences in olfactory ability among individuals of different genders.
【作者单位】：上海海洋大学鱼类研究室;上海市海洋动物系统分类与进化重点实验室;
【基金】：公益性行业(农业)科研专项(No.201203065) 国家自然科学基金项目(No.31172407,31472280) 高等学校博士学科点科研专项(No.20123104110006) 上海高校水产学高峰学科
【分类号】：S917.4

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