miR-124 rs531564、miR-26A rs7372209和miR-126 rs4636297基因多态性与结直

发布时间：2018-07-25 15:47

【摘要】：目的评估微RNA(miR)-124、miR-26A和miR-126基因单核苷酸多态性(SNP)对结直肠癌(CRC)患者预后的影响。方法以2011年7月至2013年12月该院收治的724例CRC住院患者为研究对象,利用PCR-连接酶检测反应(PCR-LDR)技术对患者外周血miR-124rs531564、miR-26Ars7372209和miR-126rs4636297进行SNPs位点基因型分析。患者的总生存时间(OS)和无复发生存率(PFS)采用Kaplan-Meier法计算,单变量log-rank法比较SNPs不同的基因型和单倍体型对OS和RFS的影响。结果携带miR-124rs531564CG基因型的CRC患者手术治疗后3年内OS较携带CC基因型的患者缩短,3年内因肿瘤死亡风险增加[HR2=1.450,95%CI(1.043~2.017),P=0.026];携带GG基因型的患者较携带CC型的患者3年内因肿瘤死亡风险亦增加[HR2=2.339,95%CI(1.171~4.674),P=0.024];在显性基因模型中,携带CG+GG基因型的患者较携带CC基因型的患者预后3年内OS明显下降[HR2=1.532,95%CI(1.119~2.096),P=0.008];隐性基因模型中,携带GG基因型与携带CC+CG基因型患者的OS无明显差异[HR2=1.975,95%CI(1.000~3.900),P=0.052];共显性模型中,以CC+CG基因型为参照,携带CG基因型的患者OS降低,患者3年内因肿瘤死亡风险增加[HR2=1.395,95%CI(1.007~1.931)]。未发现miR-26Ars7372209位点和miR-126rs 4636297位点基因多态性与CRC术后3年内OS和RFS相关(P0.05)。结论miR-124rs531564位点基因多态性是CRC患者预后的独立影响因素。
[Abstract]:Objective to evaluate the effect of RNA (miR)-124 miR-26A and miR-126 gene single nucleotide polymorphism (SNP) on the prognosis of (CRC) patients with colorectal cancer. Methods from July 2011 to December 2013, 724 patients with CRC were enrolled in this study. The SNPs genotypes of miR-124rs531564-miR-26Ars7372209 and miR-126rs4636297 in peripheral blood were analyzed by PCR-ligase assay (PCR-LDR). The total survival time (OS) and recurrence free survival rate (PFS) were calculated by Kaplan-Meier method. The effects of different genotypes and haploid types of SNPs on OS and RFS were compared by univariate log-rank method. Results the OS of CRC patients with miR-124rs531564CG genotype was shorter than that of patients with CC genotype within 3 years after operation, and the risk of tumor death was increased in 3 years [HR2A1.450 95 CI (1.043 卤2.017) P0. 026], and the patients with GG genotype died of cancer within 3 years compared with those with CC genotype. The risk of death was also increased [HR2 + 2.339% 95 CI (1.171 卤4.674) P0. 024]; in the dominant gene model, The prognosis of patients with CG GG genotype was significantly lower than that of patients with CC genotype within 3 years [HR2GG 1.532U 95CI (1.1192.096) P0. 008], there was no significant difference between patients with GG genotype and CC CG genotype in recessive gene model [HR2GG 1.97595CI (1.0003.900) P0.052]. Compared with CC CG genotype, the OS of patients with CG genotype decreased, and the risk of tumor death increased in 3 years [HR2G 1.395C _ (95) CI (1.007 ~ 1.931)]. There was no correlation between miR-26Ars7372209 locus and miR-126rs 4636297 locus polymorphism and OS and RFS within 3 years after CRC (P0.05). Conclusion Polymorphism of miR-124rs531564 locus is an independent prognostic factor in patients with CRC.
【作者单位】：江西省萍乡市人民医院检验科;
【基金】：江西省卫生和计划生育委员会基础医学研究项目(20157141)
【分类号】：R730.43;R735.34

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