甘蓝型油菜株高QTL定位及主效QTL区间候选基因预测

发布时间：2018-07-25 18:01

【摘要】：为了研究油菜株高的遗传基础,以2个甘蓝型油菜株系DH-7-9(矮杆)×DH-G-42(高杆)杂交后代连续自交的重组自交系群体(190个家系)为材料,在西宁和武汉2种环境下进行株高性状鉴定,结果显示,该重组自交系群体的株高表现连续变异并且符合正态分布。利用前期构建的遗传连锁图,结合2种环境下株高性状鉴定数据,采用Win QTLcart 2.5软件复合区间作图法(CIM)进行QTL定位和效应估计,结果表明,在2种环境下共检测到11个与株高性状相关的QTL,单个QTL可解释的表型变异为1.17%~10.45%。在A10连锁群上,主效QTL(q PH-X-A10或q PHW-A10)在两环境下可重复检测到,可解释10.24%~10.45%的表型变异。将156个拟南芥株高基因与该主效QTL置信区间对应的油菜基因组上的723个基因进行同源比较分析,在主效QTL区域内预测到2个株高候选基因Bna A10g07740D和Bna A10g12020D,其对应的拟南芥同源基因分别为ATGA20ox2、GA5/ATGA20ox1和STA1,均与拟南芥株高相关。
[Abstract]:In order to study the genetic basis of rapeseed plant height, the plant height traits of the recombinant inbred lines (190 families) of 2 hybrid Brassica napus hybrid progenies (DH-7-9 dwarf) and DH-G-42 (high pole) hybrid progeny were identified in 2 environments in Xining and Wuhan. The results showed that the plant height of the recombined inbred line was continuously variant and Win QTLcart 2.5 software complex interval mapping (CIM) was used to determine the location and effect of QTL, using the genetic linkage map constructed in the earlier period, and the Win QTLcart 2.5 software complex interval mapping (CIM). The results showed that in 2 environments, there was a total of QTL related to the plant height, and the individual QTL could be explained by 1.17% of the phenotypic variation. ~10.45%. in the A10 linkage group, the main effect QTL (Q PH-X-A10 or Q PHW-A10) can be repeated in the two environment to explain the phenotypic variation of 10.24%~10.45%. The homologous ratio of the 156 Arabidopsis high genes to 723 genes in the rape genome corresponding to the dominant QTL confidence interval is analyzed, and 2 strains of high phenology are predicted in the main effective QTL region. The corresponding genes of Bna A10g07740D and Bna A10g12020D, corresponding to ATGA20ox2, GA5/ATGA20ox1 and STA1, were all related to Arabidopsis thaliana plant height.
【作者单位】：中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室;
【基金】：国家自然科学基金项目(31371664) 武汉市科技攻关计划项目(2013020501010174) 中央级公益性科研院所基本科研业务费专项资金项目(1610172012001)
【分类号】：S565.4

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