饲料糖脂比对吉富罗非鱼生长、血液指标和肝脏糖代谢关键酶活性及基因表达的影响

发布时间：2018-07-27 10:51

【摘要】：为探讨糖脂比对吉富罗非鱼幼鱼生长性能、血液生化指标和肝脏糖代谢关键酶的影响,本实验设计了5种不同糖脂比(1.5、2.3、3.9、7.0、16.5)的等氮等能饲料,以吉富罗非鱼幼鱼为实验对象,进行了为期8周的饲养实验。结果显示,随饲料中糖脂比升高,吉富罗非鱼特定生长率(SGR)、增重率(WGR)和蛋白质效率(PER)先升高后降低,糖脂比为3.9和7.0时,WGR、SGR和PER最高。饵料系数(FCR)的变化趋势与此相反,在糖脂比为3.9和7.0组显著低于其他组。随着饲料糖脂比的增加,吉富罗非鱼全鱼粗脂肪含量逐渐下降,在糖脂比为16.5时达到最低水平,但仅与1.5组存在显著差异。血浆甘油三酯在糖脂比最高组也出现显著降低,血浆胆固醇同样在糖脂比最高组显著低于3.9组。然而血糖的变化趋势相反,当糖脂比为16.5时显著高于其他组。随着糖脂比的升高,吉富罗非鱼幼鱼肝脏中丙酮酸激酶(PK)的活性及mRNA表达量逐渐升高,而磷酸烯醇式丙酮酸羧激酶(PEPCK)的活性及mRNA表达水平则逐渐下降。根据二次回归模型得出,在等氮等能的饲料条件下,吉富罗非鱼幼鱼最适宜的糖水平和脂肪水平分别为49.69%和9.53%,其对应的糖脂比为8.4。
[Abstract]:In order to study the effects of sugar / lipid ratio on growth performance, blood biochemical indexes and key enzymes of hepatic glucose metabolism of juvenile Gifeto tilapia, five kinds of iso-nitrogen isoenergy feed with different ratio of sugar to lipid (1.5C 2.3C 3.9C 7.0N 16.5) were designed. The juvenile Tilapia japonica was used as the experimental object. A feeding experiment was conducted for 8 weeks. The results showed that the specific growth rate (SGR),) weight gain rate (WGR) and protein efficiency (PER) of tilapia japonicus increased first and then decreased with the increase of the ratio of glucose to lipid in diet. When the ratio of sugar to fat was 3.9 and 7.0, the (WGR) and PER of Tilapia japonicus were the highest. The variation trend of feed coefficient (FCR) was opposite, which was significantly lower than that of other groups when the ratio of sugar to lipid was 3. 9 and 7. 0. With the increase of the ratio of feed sugar to fat, the crude fat content of the whole fish decreased gradually and reached the lowest level at the ratio of sugar to fat of 16.5, but there was only a significant difference between the two groups. Plasma triglycerides were also significantly decreased in the highest glucose to lipid ratio group, and plasma cholesterol was also significantly lower in the highest glucose to fat ratio group than in 3.9 group. However, the change trend of blood sugar was opposite, when the ratio of sugar to fat was 16.5, it was significantly higher than that of other groups. The activity of pyruvate kinase (competitive) and the expression of mRNA increased with the increase of the ratio of glucose to lipid, but the activity of phosphoenolpyruvate carboxykinase (PEPCK) and the level of mRNA expression decreased gradually. According to the quadratic regression model, under the condition of equal nitrogen and equal energy, the optimum sugar level and fat level of juvenile tilapia were 49.69% and 9.53, respectively, and the ratio of sugar to fat was 8.4.
【作者单位】：农业部热带亚热带水产资源利用与养殖重点实验室;河南师范大学水产学院;河南省水产动物养殖工程技术研究中心;
【基金】：农业部热带亚热带水产资源利用与养殖重点实验室开放基金河南省高校科技创新团队支持计划(14IRTSTHN013) 现代农业产业技术体系专项(CARS-49)~~
【分类号】：S965.125

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