干旱胁迫下喷施甲基环丙烯对苗期甘蔗SoMAPK4基因表达的影响

发布时间：2018-07-27 13:50

【摘要】：研究甲基环丙烯(1-MCP)对甘蔗苗期叶片促分裂原活化蛋白激酶基因表达的影响,为研究1-MCP对甘蔗苗期抗旱影响提供参考。结果表明,在正常供水情况下,甘蔗So MAPK4基因在转录水平上的表达量不受1-MCP一次处理影响,而二次处理后SoMAPK4表达量呈下调趋势。干旱胁迫时,1-MCP对甘蔗So MAPK4基因在转录水平上的表达量呈先增后减、协同增减的动态变化趋势,且So MAPK4基因表达量上升比较明显,第5天达到最大值。二次处理后So MAPK4表达量出现明显增加,随干旱胁迫时间增加而逐渐减弱。由此说明,在干旱胁迫下1-MCP二次处理,能够增加甘蔗So MAPK4基因在转录水平上的表达量,且随干旱胁迫时间增加,So MAPK4基因表达量在转录水平上增加。
[Abstract]:To study the effect of methylcyclopropene (1-MCP) on the expression of mitogen-activated protein kinase gene in sugarcane leaves at seedling stage, and to provide a reference for studying the effect of 1-MCP on drought resistance in sugarcane seedling stage. The results showed that under normal water supply, the expression of so MAPK4 gene in sugarcane was not affected by the primary treatment of 1-MCP at the transcriptional level, but the expression of so MAPK4 was down-regulated after the second treatment. Under drought stress, the expression of so MAPK4 gene in sugarcane increased first and then decreased, and increased and decreased synergistically. The expression of so MAPK4 gene increased obviously and reached the maximum on the 5th day. After secondary treatment, the expression of so MAPK4 increased significantly, and gradually decreased with the increase of drought stress time. The results showed that the secondary treatment of 1-MCP under drought stress could increase the expression of so MAPK4 gene at the transcriptional level in sugarcane, and increase the expression of so MAPK4 gene at the transcriptional level with the increase of drought stress time.
【作者单位】：广西农业科学院甘蔗研究所;广西农业科学院;
【基金】：国家自然基金项目(31460093) 广西科学研究与技术开发计划项目(桂科攻1598006-1-1D,桂科AB16380258) 广西农科院科技发展基金项目(桂农科2015JM05)
【分类号】：S566.1

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