水稻抗性基因Pi对福建省稻瘟病菌优势菌群的抗性分析

发布时间：2018-07-29 06:55

【摘要】：【目的】抗病品种的合理利用和布局是实现水稻抗瘟持久化的关键因子之一。为明确水稻24个抗性基因在福建省的抗病性及其应用前景,【方法】首先分别采用全国稻瘟病菌生理小种鉴别品种和CO39近等基因系来鉴定2012-2015年间从福建省各稻区种植的普感品种丽江新团黑谷上采集的347株稻瘟病菌单孢菌株的生理小种类型和致病型,再测定24个抗性基因对福建省稻瘟病菌的抗性。【结果】根据全国稻瘟病菌生理小种鉴别品种对菌株的抗感反应,可将供试稻瘟病菌的生理小种划分为6个群36个生理小种,其中ZA、ZB和ZC为主要种群,ZC15、ZD7和ZB15为优势生理小种。根据CO39近等基因系的接种结果,将供试稻瘟病菌划分为17个致病类型,其中I34.1为优势致病型。供试的24个抗性基因对347株福建省稻瘟病菌菌株的抗性频率不同,抗谱为9.80%~89.91%。其中,Pi-k~m、Pi-7(t)、Pi-9(t)、Pi-k~p、Pi-k、Pi-k~h、Pi-z~5和Pi-ta(1)等8个抗性基因的抗病性较强,抗谱均高于70.00%。【结论】说明这8个抗性基因在福建省具有较好的应用前景,育种时可以考虑联合利用这些抗性基因。同时,实验结果表明,这8个抗性基因对主要生理小种的抗谱和主要致病型的抗谱均值高于69.00%,与其对所有测试菌株的抗谱吻合。说明利用稻瘟病菌的优势种群或优势致病型来鉴定水稻品种的抗病性是可靠的。
[Abstract]:Objective: the rational utilization and distribution of resistant varieties is one of the key factors to realize the persistence of rice blast resistance. In order to clarify the resistance of 24 rice resistance genes in Fujian Province and its application prospects, [methods] at first, 347 strains of Magnaporthe grisea Monosporium collected from Lijiang Xintuan Valley, Fujian Province from 2012 to 2015 were identified by the identification of physiological races and CO39 near isogenic lines of rice blast fungus in China from 2012 to 2015. Physiological races and pathotypes of plants, The resistance of 24 resistant genes to Magnaporthe oryzae in Fujian Province was determined. [results] according to the resistant response of physiological races of rice blast fungus in China, the physiological races of rice blast fungus could be divided into 6 groups and 36 physiological races. ZAZB and ZC were the dominant physiological races of ZC15, ZD7 and ZB15. According to the inoculation results of CO39 near isogenic lines, the tested rice blast fungus was divided into 17 pathogenic types, of which I34.1 was the dominant pathogenic type. The resistance frequency of 24 resistant genes to 347 strains of Magnaporthe grisea in Fujian Province was different, and the resistance spectrum was 9.80 and 89.91. Among them, Pi-7 (t) / Pi-9 (t) of Pi-kaphne Pi-kapu, Pi-kanhu, Pi-zni-5 and Pi-ta (1) have strong resistance to disease, and the spectrum of resistance is higher than that of 70.00.Conclusion these eight resistant genes have a good application prospect in Fujian Province, and may be considered to be used jointly in breeding. At the same time, the results showed that the mean value of the resistance spectrum of the eight resistant genes to the main physiological races and the main pathogenic types was higher than 69.00, which coincided with the resistance spectrum of the eight resistant genes to all the tested strains. It shows that it is reliable to identify the disease resistance of rice varieties by using the dominant population or the dominant pathogenic type of rice blast fungus.
【作者单位】：福建省农业科学院植物保护研究所;福建省作物有害生物监测与治理重点实验室;
【分类号】：Q786;;S435.111.4+1

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