携带rmtB基因的大肠埃希菌β-内酰胺酶基因研究
发布时间：2018-07-29 12:39
【摘要】：目的调查一组携带rmtB基因的大肠埃希菌中β-内酰胺酶基因与可移动遗传元件的存在状况,了解该组菌株之间的亲缘关系。方法收集2016年1-10月湖北医药学院附属襄阳市第一人民医院住院患者痰液标本分离到的20株携带rmtB基因的大肠埃希菌,采用K-B法测定9种抗菌药物的敏感性,采用聚合酶链反应(PCR)及序列分析法分析16种β-内酰胺酶基因和7种可移动遗传元件遗传标记;阳性耐药基因测序后直接作BLAST比对,耐药基因检测结果作样本聚类分析(UPGMA法)。结果 20株携带rmtB基因的大肠埃希菌对头孢类、氨基糖苷类、喹诺酮类均耐药,但对碳青霉烯类均敏感;20株菌均检出β-内酰胺酶基因及可移动遗传元件遗传标记;20株菌共检出5种β-内酰胺酶基因和7种可移动遗传元件遗传标记,且阳性率较高;样本聚类分析显示20株菌有明显的聚集性,分A与B二个群,并有4个克隆传播。结论本组20株携带rmtB基因的大肠埃希菌同时携带了β-内酰胺酶基因和可移动遗传元件遗传标记,是对头孢类和氨基糖苷类产生耐药的重要原因;本组菌检出的4个克隆高度疑似医院感染,同一克隆菌株携带相同基因。
[Abstract]:Objective to investigate the presence of 尾 -lactamase gene and movable genetic elements in Escherichia coli carrying rmtB gene, and to understand the relationship between these strains. Methods from January to October 2016, 20 strains of Escherichia coli carrying rmtB gene were collected from the sputum samples of the first people's Hospital of Xiangyang affiliated to Hubei Institute of Medicine. The sensitivity of 9 antimicrobial agents was determined by K-B method. Polymerase chain reaction (PCR) and sequence analysis were used to analyze 16 尾 -lactamases genes and 7 kinds of mobile genetic element genetic markers, positive drug resistance genes were sequenced and BLAST was compared directly, and the results of drug resistance gene detection were analyzed by sample cluster analysis (UPGMA method). Results 20 strains of Escherichia coli carrying rmtB gene were resistant to cephalosporins, aminoglycosides and quinolones. However, 尾 -lactamase genes were detected in all 20 strains sensitive to carbapenes, and 5 尾 -lactamases genes and 7 transportable genetic element genetic markers were detected in 20 strains with movable genetic elements, and the positive rate was higher. The sample cluster analysis showed that 20 strains had obvious aggregation, which were divided into A and B groups, and 4 clones were transmitted. Conclusion the 20 strains of Escherichia coli carrying rmtB gene carry both 尾 -lactamase gene and mobile genetic element genetic markers, which is an important cause of drug resistance to cephalosporins and aminoglycosides. Four clones were highly suspected of nosocomial infection, and the same cloned strain carried the same gene.
【作者单位】： 湖北医药学院附属襄阳市第一人民医院;宁波市第一医院检验科;
【基金】：浙江省中医药基金资助项目(2011ZB126)
【分类号】：R440
，
本文编号：2152698
[Abstract]:Objective to investigate the presence of 尾 -lactamase gene and movable genetic elements in Escherichia coli carrying rmtB gene, and to understand the relationship between these strains. Methods from January to October 2016, 20 strains of Escherichia coli carrying rmtB gene were collected from the sputum samples of the first people's Hospital of Xiangyang affiliated to Hubei Institute of Medicine. The sensitivity of 9 antimicrobial agents was determined by K-B method. Polymerase chain reaction (PCR) and sequence analysis were used to analyze 16 尾 -lactamases genes and 7 kinds of mobile genetic element genetic markers, positive drug resistance genes were sequenced and BLAST was compared directly, and the results of drug resistance gene detection were analyzed by sample cluster analysis (UPGMA method). Results 20 strains of Escherichia coli carrying rmtB gene were resistant to cephalosporins, aminoglycosides and quinolones. However, 尾 -lactamase genes were detected in all 20 strains sensitive to carbapenes, and 5 尾 -lactamases genes and 7 transportable genetic element genetic markers were detected in 20 strains with movable genetic elements, and the positive rate was higher. The sample cluster analysis showed that 20 strains had obvious aggregation, which were divided into A and B groups, and 4 clones were transmitted. Conclusion the 20 strains of Escherichia coli carrying rmtB gene carry both 尾 -lactamase gene and mobile genetic element genetic markers, which is an important cause of drug resistance to cephalosporins and aminoglycosides. Four clones were highly suspected of nosocomial infection, and the same cloned strain carried the same gene.
【作者单位】： 湖北医药学院附属襄阳市第一人民医院;宁波市第一医院检验科;
【基金】：浙江省中医药基金资助项目(2011ZB126)
【分类号】：R440
，
本文编号：2152698
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