环丙沙星对小鼠原代肝细胞基因表达谱芯片的研究

发布时间：2018-07-31 17:00

【摘要】：目的研究环丙沙星对小鼠原代肝细胞基因表达的影响。方法实验组(EG)和对照组(CG)小鼠分别灌胃给予环丙沙星和生理盐水7d提取肝细胞,采用Agilent基因表达谱芯片技术检测基因表达,对差异表达基因进行生物信息学分析,并挑选部分基因进行荧光定量PCR验证。结果 (1)实验组小鼠肝细胞中共筛选到328个差异表达基因(fold-change1.5或0.667)。(2)基因本体论(gene ontology,GO)功能分析显示差异表达基因主要富集在细胞有丝分裂周期、转移酶活性调节、催化活性调节和细胞周期调控等生物学过程;KEGG pathway分析则显示内吞、P53和HIF-1信号通路受到影响。(3)环丙沙星上调SCARA5和OSGIN1基因,下调PFKFB3和SMOC2基因,4个基因表达的荧光定量PCR与芯片测试结果相符。结论环丙沙星影响肿瘤发生发展的生物学过程,并可能通过调控SCARA5、OSGIN1、PFKFB3和SMOC2等肿瘤相关基因发挥抗肿瘤活性。
[Abstract]:Objective to study the effect of ciprofloxacin on gene expression in mouse primary hepatocytes. Methods (EG) mice in experimental group and (CG) mice in control group were given ciprofloxacin and normal saline respectively for 7 days to extract liver cells. Agilent gene expression microarray technique was used to detect gene expression, and bioinformatics analysis of differentially expressed genes was carried out. Some genes were selected for fluorescence quantitative PCR verification. Results (1) A total of 328,328differentially expressed genes (fold-change1.5 or 0.667). (2) were screened out from the hepatocytes of the experimental group. The functional analysis of gene ontology go showed that the differentially expressed genes were mainly enriched in the mitotic cycle, and the activity of transferase was regulated. KEGG pathway analysis showed that endocytosis p53 and HIF-1 signaling pathway were affected. (3) ciprofloxacin upregulated SCARA5 and OSGIN1 genes. PFKFB3 and SMOC2 genes were down-regulated. The fluorescence quantitative PCR of four genes were in agreement with the results of microarray test. Conclusion ciprofloxacin affects the biological process of tumor development and may play an antitumor role by regulating SCARA5 OSGIN1 PFKFB3 and SMOC2 genes.
【作者单位】：四川抗菌素工业研究所成都大学;
【分类号】：R965

【相似文献】