白背飞虱18S核糖体基因克隆及系统发育

发布时间：2018-08-01 08:55

【摘要】：以白背飞虱Sogatella furcifera长翅雌虫为材料,提取其基因组DNA,以已知的半翅目昆虫18S rDNA全序列和白背飞虱18S rDNA部分序列为参考,通过PCR扩增出白背飞虱18S rDNA的未知部分序列,最后通过拼接,首次得到了白背飞虱18S rDNA的全长序列,长度为1 891 bp,碱基组成比例均衡,分别为:24.1%A;23.7%C;27.7%G;24.5%T。将此全长序列与分属6个目(半翅目、双翅目、膜翅目、直翅目、鞘翅目、等翅目)昆虫的18S rDNA序列进行比较,一共发现有4个保守区域,其中第二个区域发生插入或缺失的情况最少。以18S rDNA第二保守区域进行系统发育分析,发现原同翅目昆虫多数均与半翅目昆虫菜蝽亲缘关系较近,但原同翅目飞虱科昆虫,如白背飞虱、褐飞虱则与半翅目昆虫菜蝽的亲缘关系较远,表明近年来将原同翅目昆虫全部划归为半翅目还有待商榷之处。
[Abstract]:The genomic DNAs of Sogatella furcifera longwinged female were extracted. The unknown sequence of 18s rDNA was amplified by PCR with reference to the known 18s rDNA sequence of Hemiptera and the partial sequence of 18s rDNA of Whitebacked planthopper. Finally, the complete length of 18s rDNA was obtained for the first time. The length of 18s rDNA was 1 891bp. the proportion of the base composition was balanced, which was: 1: 24.1A ~ (23.7A) and C27.7G ~ (24.5T), respectively. The full-length sequence was compared with the 18s rDNA sequence of 6 orders (Hemiptera, Diptera, Hymenoptera, Orthoptera, Coleoptera, Isoptera). The second area is least likely to be inserted or missing. Phylogenetic analysis of the second conservative region of 18s rDNA showed that most of the homoptera insects were closely related to the hemiptera insects, but the homoptera insects, such as the white back planthopper, were closely related to each other. The phylogenetic relationship between Brown planthopper and Hemiptera insects is far from that of Hemiptera, indicating that the classification of Prohomoptera as Hemiptera in recent years is still open to question.
【作者单位】：中山大学有害生物控制与资源利用国家重点实验室//昆虫学研究所;
【基金】：广东省自然科学基金(S2022020002353,031628) 国家自然科学基金(311771844)
【分类号】：S435.112.3

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