辽棉19号转化耐盐基因AlNHX1的研究

发布时间：2018-08-02 18:27

【摘要】：为提高辽宁棉花品种的耐盐性,利用农杆菌介导上胚轴外植体转化法,将来源于盐生植物獐茅的Na~+/H~+逆向转运蛋白基因(AlNHX1)导入棉花辽棉19号中,分析影响棉花上胚轴外植体农杆菌转化的几个重要因素,建立并优化了该品种的转化体系,结果表明:1)农杆菌侵染的最佳时间为90s,共培养过程中乙酰丁香酮最适浓度为100 mg/L,筛选培养基中潮霉素最适浓度为30 mg/L,生根培养基中吲哚乙酸IBA的最适浓度为10mg/L;2)对转化植株进行PCR检测,表明耐盐基因AlNHX1已导入辽棉19号中;3)在盐胁迫下,转基因植株的电导率,渗透势都显著低于未转化植株。结果表明,通过筛选并优化转化体系,辽棉19号品种的耐盐性有较明显的提升,为沿海滩涂地区种植棉花提供了一定的参考。
[Abstract]:In order to improve the salt tolerance of cotton varieties in Liaoning Province, the Na- / H- antiporter gene (AlNHX1), which was derived from the halophyte, was introduced into cotton Liaomian19 by Agrobacterium tumefaciens (Agrobacterium tumefaciens) mediated transformation of epicotyl explants. Several important factors affecting the transformation of Agrobacterium tumefaciens in cotton epicotyl explants were analyzed, and the transformation system of this variety was established and optimized. The results showed that the optimal infection time of Agrobacterium tumefaciens was 90 s, the optimal concentration of acetyl eugenone was 100 mg / L, the optimum concentration of hygromycin was 30 mg / L and the optimal concentration of IBA in rooting medium was 10 mg / L ~ (2). The transformed plants were detected by PCR. The results showed that the salt tolerance gene AlNHX1 had been introduced into Liaomian19 Zhongni3) under salt stress, the conductivity and osmotic potential of the transgenic plants were significantly lower than those of the untransformed plants. The results showed that through screening and optimizing the transformation system, the salt tolerance of Liaomian19 was obviously improved, which provided a certain reference for planting cotton in coastal shoal area.
【作者单位】：辽宁省农业科学院大连生物技术研究所;
【基金】：辽宁省自然科学基金(2014027023)
【分类号】：S562

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