牛樟芝菌丝体和子实体三萜含量测定及Se和Mvd基因表达分析

发布时间：2018-08-04 18:52

【摘要】：为探讨牛樟芝子实体和不同培养时期的菌丝体中三萜含量及鲨烯环氧酶基因(Se)和甲羟戊酸焦磷酸脱羧酶基因(Mvd)的表达情况,采用香草醛-冰醋酸法测定菌丝体和子实体的三萜含量,并利用实时荧光定量PCR(qRT-PCR)分析甲羟戊酸途径(MVA)中Se和Mvd基因表达水平。结果表明,随着培养时间的推移,当生长至21 d时牛樟芝菌丝体干重逐渐趋于稳定。当培养时间小于28 d时,菌丝体中三萜含量随着培养时间的延长而逐渐增加,且28 d时菌丝体的三萜含量最高,达(39.192±2.025)mg/g,仅次于牛樟芝子实体三萜含量(49.391±2.675)mg/g,玉米芯栽培的牛樟芝子实体三萜含量最低,为(11.530±0.733)mg/g,各样品间三萜含量差异达到显著水平(P0.05)。qRT-PCR分析结果表明,培养7 d的菌丝体中Se和Mvd的表达量均为最高,但随着培养时间的继续延长而呈现下降趋势;牛樟芝子实体中Se和Mvd的表达量均较低,与培养28~42 d的菌丝体表达量相当。本研究结果表明牛樟芝的三萜含量可能与其合成途径中相关基因的表达量、培养条件和累积时间有关。
[Abstract]:In order to study the triterpene content in the fruiting body and mycelium of Coyoderma lucidum and the expression of squalene epoxylase gene (Se) and (Mvd) in the mycelium of different culture period, The triterpenoid content of mycelium and fruiting body was determined by vanillin-glacial acetic acid method. The expression of se and Mvd genes in (MVA) via mevalic acid pathway was analyzed by real-time fluorescence quantitative PCR (qRT-PCR). The results showed that the dry weight of mycelium tended to be stable at 21 days with the passage of culture time. When the culture time was less than 28 days, the triterpenoid content of mycelia increased with the extension of culture time, and the triterpenoid content of mycelium was the highest at 28 days. The content of triterpenoids was (39.192 卤2.025) mg / g, which was only (49.391 卤2.675) mg / g in the fruiting body of Camphor lucidum, and the lowest (11.530 卤0.733) mg / g in corncob. The difference of triterpene content among the samples was significant (P0.05) .qRT-PCR analysis showed that the content of triterpenoid in the fruiting body of the corncob was (11.530 卤0.733) mg / g. The expression of se and Mvd was the highest in mycelia cultured for 7 days, but decreased with the prolongation of culture time, and the expression of se and Mvd in the fruiting body of Bos camphora was lower than that of the mycelia cultured for 2842 days. The results suggested that the triterpenoid content of Acanthus camphora might be related to the expression of related genes in its synthesis pathway, culture conditions and accumulation time.
【作者单位】：国家菌草工程技术研究中心;福建农林大学生命科学学院;福建省农业科学院果树研究所;
【基金】：福建省菌草生态产业协同创新中心(K80ND8002) 福建农林大学科技发展基金(KF2015111) 国家菌草工程技术研究中心组建项目(2011FU125X12)
【分类号】：S567.3

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